AtT20

87021902, mouse brain, Small round cells growing as large aggregates

• UNSPSC Code: 41106514

Properties

• Product Name: AtT20, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 87021902
• biological source: mouse brain
• packaging: tube of 5 μg 87021902-DNA-5UG; pkg of vial of cells 87021902-1VL
• growth mode: Aggregates in suspension
• karyotype: Diploid
• morphology: Small round cells growing as large aggregates
• products: Adrenocorticotropic hormone (ACTH)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse LAF1 pituitary gland tumour

Cell Line Description

Derived after alternate passage of LAF1 mouse pituitary tumour cells as tumours in animals and in cell culture. Cells produce adrenocorticotropic hormone (ACTH) at approximately the rate of 1000mU/mg of cell protein per week.

Application

Hormone studies

Culture Medium

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). Cells grow in large aggregates.

Subculture Routine

Allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. A subcultivation ratio of 1:2 is recommended. Cells grow in large viable clusters making counting difficult. A seeding densi

Other Notes

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