A2230

Apyrase from potatoes

High Activity, ATPase ≥600 units/mg protein, lyophilized powder

• Synonym(s): Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase
• CAS Number: 9000-95-7
• Enzyme Commission number: 3.6.1.5 (BRENDA, IUBMB)
• MDL number: MFCD00130542
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: potato
• Quality Level: 300
• form: lyophilized powder
• quality: High Activity
• ATPase activity: ≥600 units/mg protein
• secondary activity: ≥50 % of base activity ADPase
• composition: protein, ≥30%
• foreign activity: Acid Phosphatase ≤2% of base activity
• shipped in: wet ice
• storage temp.: −20°C

Description

Application

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation . Product A2230 is a high activity apyrase.

At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+P_i → AMP+2P_i.

Biochem/physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Packaging

Sold on the basis of ATPase units.

Unit Definition

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Physical form

Lyophilized powder containing potassium succinate buffer salts.

Preparation Note

Derived from red potato

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable