Skip to Content
Merck
All Photos(2)

Key Documents

91A

Sigma-Aldrich

Leukocyte α-Naphthyl Acetate (Non-Specific Esterase) Kit

Kit formulated with all liquid reagents.

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
41116123
NACRES:
NA.47

Quality Level

form

liquid

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

application(s)

hematology
histology

shipped in

wet ice

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Application

Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates. According to the methodology, α-naphthyl acetate is enzymatically hydrolyzed, liberating a free naphthol compound. This then couples with a diazonium compound, forming black colored deposits at sites of non-specific esterase activity. This enzyme is detected primarily in monocytes, macrophages and histocytes, and is normally absent in granulocytes. Lymphocytes may occasionally exhibit enzyme activity. A sodium fluoride inhibition procedure may be run to demonstrate monocyte inhibition. The procedure is run at 37 °C and may be utilized with blood, bone marrow films, tissue-touch preparations, cytocentrifuge preparations and frozen sections. The enzyme does not survive paraffin processing.

Kit Components Only

Product No.
Description

  • TRIZMAL™ 7.6 Concentrate 50 mL

  • Hematoxylin solution gill no. 3 50 mL

Kit Components Also Available Separately

Product No.
Description
SDS

  • 916α-Naphthyl Acetate Solution 10 mLSDS

  • 915Citrate Solution, pH 3.6±0.1 (25 °C), 27 mM 50 mLSDS

  • 917Fast Blue BB Base Solution 10 mLSDS

  • 914Sodium nitrite solution 10 mLSDS

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - STOT SE 1

Target Organs

Eyes,Central nervous system

Storage Class Code

3 - Flammable liquids

Flash Point(F)

98.1 °F

Flash Point(C)

36.7 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service