69964

MRS Agar

suitable for microbiology, NutriSelect^® Basic

• Synonym(s): Lactobacillus Agar according to DeMan, Rogosa and Sharpe
• UNSPSC Code: 41171605
• NACRES: NA.74

Properties

• sterility: non-sterile
• Quality Level: 100
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 12 g/L ; dipotassium hydrogen phosphate, 2 g/L ; D(+)-glucose, 20 g/L ; magnesium sulfate (equivalent to 0.2 g/lheptahydrate), 0.1 g/L ; manganous sulfate monhydrate, 0.05 g/L ; meat extract, 5 g/L ; sodium acetate, 5 g/L ; triammonium citrate, 2 g/L ; universal peptone, 10 g/L ; yeast extract, 5 g/L
• manufacturer/tradename: NutriSelect^® Basic
• technique(s): microbiological culture: suitable
• final pH: 6.5±0.2 (25 °C)
• application(s): agriculture; bioburden testing; environmental; food and beverages; microbiology
• suitability: nonselective for Lactobacillus spp.; selective by low pH for Bifidobacterium spp.

Description

Application

For the enrichment, cultivation and isolation of all species of Lactobacillus from all types of material

Preparation Note

Suspend 61.15 g in 1000 ml distilled water containing 1 ml polysorbate 80 (TWEEN^® 80; Sigma P8074). Boil to dissolve the medium completely and mix thoroughly. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If necessary adjust the pH with glacial acetic acid after sterilization.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves