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UFC8010

Millipore

Amicon® Ultra Centrifugal Filter, 10 kDa MWCO

sample volume 4 mL, regenerated cellulose membrane, MWCO 10 kDa

Synonym(s):

Centrifugal concentrator, 10 kDa MWCO, 4 mL sample volume

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About This Item

UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24

material

polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing

Quality Level

sterility

non-sterile

product line

Amicon®

manufacturer/tradename

Amicon® Ultra

parameter

4 mL sample volume

technique(s)

protein extraction: suitable
protein purification: suitable

L

124 mm (4.88 in.)

diam.

17.3 mm

filtration area

3 cm2

volume

4 mL

pore size

10 kDa MWCO

shipped in

ambient

General description

Amicon® Ultra 4 mL centrifugal devices with Ultracel® 10,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Cytochrome C (12.4 kDa) at 0.25 mg/ml initial concentration is retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.

Application

  • Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane.
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
  • Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
  • Concentration of dilute or pre-purified proteins from column eluents.
  • Desalting, buffer exchange and protein dialysis.
  • Size exclusion and protein fractionation.

Features and Benefits

  • Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%.
  • Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).
  • Heat-sealed membrane minimizes downstream extractables.
  • 100% integrity tested for reliable performance.
  • Convenient sample monitoring with translucent housing and volume gradations.
  • Direct pipette sample access eliminates processing step to recover concentrate.
  • High concentration factors of 80–100X.

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10-13 - German Storage Class 10 to 13


Certificates of Analysis (COA)

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Protocols

Accurate measurement of specific proteins in urine is important for diagnosing and managing diseases.

Using a standard Amicon® Ultra centrifugal filter, a few rounds of diafiltration can efficiently remove unincorporated label from an antibody labeling reaction without diluting the sample.

Biomarkers play an essential role in the drug discovery and development process.

Biomarkers play an essential role in the drug discovery and development process.

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