371208
Dkk-1, Rat, Recombinant
Dkk-1, Rat, Recombinant, consists of amino acids 23-270, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells.
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.27
recombinant
expressed in mouse NSO cells
Quality Level
Assay
≥95% (SDS-PAGE)
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤1.0 EU/μg protein Endotoxin
shipped in
ambient
storage temp.
−20°C
General description
Dkk-1, Rat, Recombinant, consists of amino acids 23-270, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells.
Recombinant, rat Dickkopf related protein-1 (Dkk-1), consisting of amino acids 23-270, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells. The balance between Wnt signaling and Dkk-1 inhibition is essential for bone formation and homeostasis.
Biochem/physiol Actions
ND₅₀ = 0.1-0.5 µg/ml as measured by inhibition of Wnt-3a-induced (10 ng/ml rmWnt-3a) alkaline phosphatase production by proliferation of MC3T3-E1 mouse osteoblastic cells.
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from 0.2 µm filtered PBS, BSA.
Reconstitution
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.
Other Notes
Li, J. et al. 2006. Bone36, 754.
Morvan, F. et al. 2006. J. Bone Miner. Res.21, 934.
Niehrs, C. 2006. Oncogene25, 7469.
Verani, R. et al. 2006. J. Neurochem.101, 242.
Cappuccio, I. et al. 2005. J. Neurosci.25, 2647.
Kemp, C. et al. 2005. Dev. Dyn.233, 1064.
Adamska, M. et al. 2004. Dev. Biol.272, 134.
Bullock, C.M. et al. 2004. Mol. Pharmacol.65, 582.
Mao, B. et al. 2002. Nature417, 664.
Mao, B. et al. 2001. Nature411, 321.
Krupnik, V.E., et al. 1999. Gene238, 301.
Morvan, F. et al. 2006. J. Bone Miner. Res.21, 934.
Niehrs, C. 2006. Oncogene25, 7469.
Verani, R. et al. 2006. J. Neurochem.101, 242.
Cappuccio, I. et al. 2005. J. Neurosci.25, 2647.
Kemp, C. et al. 2005. Dev. Dyn.233, 1064.
Adamska, M. et al. 2004. Dev. Biol.272, 134.
Bullock, C.M. et al. 2004. Mol. Pharmacol.65, 582.
Mao, B. et al. 2002. Nature417, 664.
Mao, B. et al. 2001. Nature411, 321.
Krupnik, V.E., et al. 1999. Gene238, 301.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
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