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Protein G Agarose, Fast Flow

Protein G Agarose, Fast Flow suitable for medium and low pressure chromatography of IgG from mouse, sheep, and rabbit, and for immunoprecipitations.

Synonym(s):

Protein G resin

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About This Item

UNSPSC Code:
41116133
eCl@ss:
32160801
NACRES:
NA.56

form

liquid

manufacturer/tradename

Upstate®

technique(s)

affinity chromatography: suitable
immunoprecipitation (IP): suitable
western blot: suitable

shipped in

wet ice

General description

Fast flow agarose comprises highly crosslinked agarose beads or gels that can tolerate high pressures compared to standard agarose bead resins. Thus, these resins are ideal for fast protein liquid chromatography (FPLC) purification methods. They are preferred for medium to large-scale antibody purification. This product contains protein G covalently coupled by cyanogen bromide to highly cross-linked 4% agarose beads. It is stable in all aqueous buffers used in protein G chromatography.

Application

Protein G Agarose, Fast Flow has been used in immunoprecipitation. It is also suitable for medium and low-pressure chromatography of IgG from mice, sheep, and rabbits.

Quality

routinely evaluated by immunoprecipitation and purification

Physical form

water containing 20% ethanol

Storage and Stability

1 year at 4°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

116.6 °F

Flash Point(C)

47 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hyper-activation of TGF-β signaling is critically involved in progression of hepatocellular carcinoma (HCC). However, the events that contribute to the dysregulation of TGF-β pathway in HCC, especially at the post-translational level, are not well understood. Associations of deubiquitinase POH1 with
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PloS one, 9(10), e110514-e110514 (2014-10-14)
Changes in ion distribution across skeletal muscle membranes during muscle activity affect excitability and may impair force development. These changes are counteracted by the Na,K-ATPase. Regulation of the Na,K-ATPase is therefore important for skeletal muscle function. The present study investigated
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