Skip to Content
Merck
All Photos(1)

Documents

12-338

Sigma-Aldrich

Wortmannin, (PI3 Kinase inhibitor)

The Wortmannin, (PI3 Kinase inhibitor) controls the biological activity of PI3 Kinase. This small molecule/inhibitor is primarily used for Biochemicals applications.

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.41

Quality Level

Assay

≥95% (HPLC)

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

UniProt accession no.

shipped in

wet ice

General description

Wortmannin, a fungal metabolite, is a cell-permeable, irreversible inhibitor of phosphatidylinositol 3 kinase (PI3 Kinase) with an IC50 of 5nM. It blocks the catalytic activity of PI3-Kinase without affecting upstream signaling events such as insulin receptor tyrosine kinase activity. Wortmannin also inhibits the activities of myosin light chain kinase and PI4-Kinase at concentrations a hundred times higher than those required to inhibit PI3-Kinase.

Biochem/physiol Actions

Inhibitor Type: Kinase
Protein Target: PI3Kγ
Target Sub-Family: PI3K/PI4K

Quality

Routinely evaluated by kinase assay.
Purity: ≥95% by HPLC.
Molecular Formula: C23H24O8
Molecular Weight: 428.4

Physical form

1mg of lyophilized powder packaged under inert gas. White to off-white powder.

Storage and Stability

Protect from light. Stable for 2 years as supplied at -20°C. Following reconstitution aliquot and store at -20°. The DMSO/ethanol stock solutions are stable for 1-3 months at -20°C.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Skull and crossbones

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 1 Oral - Acute Tox. 2 Dermal

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Essential role of phosphatidylinositol 3-kinase in insulin-induced glucose transport and antilipolysis in rat adipocytes. Studies with a selective inhibitor wortmannin
Okada, T, et al
The Journal of Biological Chemistry, 269, 3568-3573 (1994)
M J Cross et al.
The Journal of biological chemistry, 270(43), 25352-25355 (1995-10-27)
Wortmannin and its structural analogue demethoxyviridin (DMV) have been reported to be specific inhibitors of phosphatidylinositol 3-kinase activity. Here we report that these compounds are not as selective as assumed and demonstrate inhibition of bombesin-stimulated phospholipase A2 activity by both
Platelet-derived growth factor-induced phosphatidylinositol 3-kinase activation mediates actin rearrangements in fibroblasts.
Wymann, M and Arcaro, A
The Biochemical Journal, 298 Pt 3, 517-520 (1994)
Wortmannin inhibits mitogen-activated protein kinase activation induced by platelet-activating factor in guinea pig neutrophils.
Ferby, I M, et al.
The Journal of Biological Chemistry, 269, 30485-30488 (1994)
Wortmannin, a specific inhibitor of phosphatidylinositol-3 kinase, blocks osteoclastic bone resorption.
Nakamura, I, et al.
Febs Letters, 361, 79-84 (1995)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service