59164
SUPELCOSIL™ LC-18-DB (5 µm) HPLC Columns
L × I.D. 30 cm × 4 mm, HPLC Column
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product name
SUPELCOSIL™ LC-18-DB HPLC Column, 5 μm particle size, L × I.D. 30 cm × 4 mm
Agency
suitable for USP L1
Quality Level
feature
endcapped
manufacturer/tradename
SUPELCOSIL™
extent of labeling
11.0% Carbon loading
parameter
0-70 °C temperature
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
30 cm × 4 mm
surface area
170 m2/g
surface coverage
surface coverage 3.1 μmol/m2
matrix
silica gel, spherical particle platform
matrix active group
C18 (octadecyl) phase
particle size
5 μm
pore size
120 Å
application(s)
food and beverages
separation technique
reversed phase
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General description
SUPELCOSIL LC-DB phases are specially deactivated for basic compounds. These columns provide shorter retention, better peak shape, and higher efficiency for organic bases than can be obtained on other Type A silica reversed-phase columns.
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
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Analytical biochemistry, 199(1), 125-131 (1991-11-15)
A rapid, isocratic method for the determination of tryptophan in Escherichia coli fermentation broths by reversed-phase HPLC is described. Tryptophan can be measured in fermentations containing either chemically defined media or media with hydrolyzed protein supplements. The procedure was rugged
Journal of chromatography. B, Biomedical sciences and applications, 755(1-2), 331-335 (2001-06-08)
A HPLC-UV determination of loratadine in human plasma is presented. After simple liquid-liquid extraction with 2-methylbutane-hexane (2:1) and evaporation of organic phase the compounds were re-dissolved in 0.01 M HCl, evaporated again and finally separated on a Supelcosil LC-18-DB column.
Journal of pharmaceutical and biomedical analysis, 26(4), 637-650 (2001-08-23)
An LC/MS/MS method for the simultaneous determination of cilostazol, a quinolinone derivative, and three active metabolites, OPC-13015, OPC-13213, and OPC-13217, in human plasma was developed and validated. Cilostazol, its metabolites, and the internal standard, OPC-3930 were extracted from human plasma
Clinical chemistry, 34(12), 2502-2503 (1988-12-01)
A high-performance liquid chromatographic (HPLC) method for determining verapamil and norverapamil in plasma is presented and compared with gas chromatography/mass spectrometry (GC-MS). The plasma samples were extracted at alkaline pH with hexane containing 2-butanol (20 mL/L) and then back-extracted into
Journal of pharmaceutical and biomedical analysis, 18(1-2), 201-211 (1998-12-24)
A study was undertaken for the characterization and quantitative determination of the main urinary metabolites of the non-steroidal anti-inflammatory drug (NSAID) nimesulide (4-nitro-2-phenoxy-methanesulfonanilide) in man following single oral administration (200 mg). Urines were collected from six healthy volunteers at 12
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