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T8575

Sigma-Aldrich

Anti-Tryptophan Hydroxylase Antibody

sheep polyclonal

Synonym(s):

Anti-TPH

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About This Item

MDL number:
MFCD00282500
UNSPSC Code:
12352203
NACRES:
NA.41

product name

Anti-Tryptophan Hydroxylase antibody produced in sheep, affinity isolated antibody, buffered aqueous glycerol solution

biological source

sheep

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

usage

sufficient for 10 blots

species reactivity

mammals, human

technique(s)

western blot: 1:1,000 using human dorsal raphe nucleus

UniProt accession no.

P17752

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TPH1(7166)
mouse ... Tph1(21990)
rat ... Tph1(24848)

General description

The tryptophan hydroxylase 1 (TPH1) gene spanning 29kbp with 11 exons is mapped to human chromosome 11p15.3-p14. TPH1, is a 444 amino acid protein expressed in the gut, spleen, thymus and also in the pineal gland and the pituitary.
Tryptophan Hydroxylase (TPH) is a member of the aromatic amino acid hydroxylases (AAAH) family.

Immunogen

recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.

Application

Anti-Tryptophan Hydroxylase antibody produced in sheep has been used in:
  • immunocytochemistry
  • immunohistochemistry
  • immunofluorescence
  • immunoperoxidase staining

Biochem/physiol Actions

The tryptophan hydroxylase 1 gene (TPH1) catalyzes the synthesis of 5-hydroxytryptophan, which is a precursor to the neurotransmitter serotonin. Variation in the gene expression affects prefrontal cortex activation during response inhibition. Mutation in the TPH1 gene leads to schizophrenia, irritable bowel syndrome (IBS) in women, suicidal behavior and depressive disorders. The encoded protein plays a vital role in regulation of cardiovascular function.

Physical form

Solution in 150 mM NaCl, 10 mM HEPES, pH 7.5, 100 μg per mL BSA, and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Matthew W Hale et al.
Experimental neurology, 224(1), 271-281 (2010-04-13)
Serotonin plays an important role in the regulation of anxiety states and physiological responses to aversive stimuli. Intracerebroventricular (i.c.v.) injection of the stress- and anxiety-related neuropeptide urocortin 2 (Ucn 2) increases c-Fos expression in serotonergic neurons in the dorsal (DRD)
Mahsa Moaddab et al.
Scientific reports, 10(1), 18035-18035 (2020-10-24)
Early adolescent adversity increases adult risk for anxiety disorders. The ventrolateral periaqueductal gray (vlPAG) and neighboring dorsal raphe (DR) are integral to threat prediction, and are responsive to acute stressors. Here, we tested the hypothesis that early adolescent adversity reshapes
GABAergic neurons in the dorsal raphe nucleus that express 5-HT3A receptors participate in responses to stress hormones
Li X, et al.
Neuroscience, 9(7), e101420-e101420 (2020)
Phylogenetic analysis and evolution of aromatic amino acid hydroxylase
Cao J, et al.
Febs Letters, 584(23), 4775-4782 (2010)
Leah R Brooks et al.
Behavioural brain research, 264, 74-81 (2014-02-12)
Serotonergic neurons in the dorsal raphe nucleus (DR) are organized in anatomically distinct subregions that form connections with specific brain structures to modulate diverse behaviors, including anxiety-like behavior. It is unclear if the functional heterogeneity of these neurons is coupled
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