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SRP0736

Sigma-Aldrich

PP2A ALPHA/PPP2R1A Complex, Active

recombinant

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

Pricing and availability is not currently available.

recombinant

expressed in baculovirus infected Sf9 cells

Assay

70-95% (SDS-PAGE)

form

frozen liquid

specific activity

3400-4600 nmol/min·mg (BIOMOL Green)

mol wt

~38 kDa
~65 kDa

concentration

0.1 μg/μL

accession no.

NM_002715
NM_014225

shipped in

dry ice

storage temp.

−70°C

Gene Information

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This Item
SAB4200742SAB4503660WH0004609M2
biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

mouse

antibody form

affinity purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

technique(s)

ELISA: 1:1,000- 1:30,000, immunocytochemistry: 1:100-1:400, western blot: 1:1,000- 1:30,000, ELISA: 1:100- 1:500 (for coating plates), immunoprecipitation (IP): 1- 4 μg/mg

technique(s)

immunoblotting: 1:250-1:500 using lysate of HEK-293T cells over expressing c-Myc fusion protein, immunohistochemistry: suitable

technique(s)

ELISA: 1:40000, immunofluorescence: 1:100-1:500, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect ELISA: suitable, western blot: 1-5 μg/mL

Application

Useful for Phosphatase assay and Western blot.

Biochem/physiol Actions

Recombinant full length human PP2Aalpha and human PPP2R1A were expressed by Baculovirus in Sf9 insect cells using an N-terminal His tag. PP2Aalpha is also known as Protein phosphatase 2A active subunit. PP2A is one of the four major Ser/Thr phosphatases and is a human tumor suppressor that inhibits cellular transformation. PP2A alpha gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase-2A (1). Protein phosphatase 2A holoenzyme is a heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. PTP2A alpha can alter the substrate specificity of PP2A leading to enhanced phosphotyrosine phosphatase activity and decreased phosphoserine phosphatase activity (2).

Reconstitution

Formulated in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.

Storage and Stability

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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