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S3652

Sigma-Aldrich

Shields and Sang M3 Insect Medium

With ʟ-glutamine and potassium bicarbonate, liquid, sterile-filtered, suitable for insect cell culture

Synonym(s):

Insect Medium, M3 Insect Medium, Sang Insect Medium

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About This Item

MDL number:
MFCD00243218
UNSPSC Code:
12352207
NACRES:
NA.75

product name

Shields and Sang M3 Insect Medium, With L-glutamine and potassium bicarbonate., liquid, sterile-filtered, suitable for insect cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | insect: suitable

components

glucose: 10 g/L (Dextro)
L-glutamine: 0.6 g/L

shipped in

ambient

storage temp.

2-8°C

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Application

Shields and Sang M3 Insect Medium has been used to culture Drosophila cell lines, larvae, and testes. 
Developed for the culture of Drosophila cell lines.

Biochem/physiol Actions

Insect tissue culture media have been formulated to mimic the main properties of the hemolymph of specific insects. A survey of the formulas of these media reveals significant differences in their composition. When supplemented with 5-20% heat-inactivated fetal bovine serum, Shields and Sang M3 medium has been found to support the rapid growth of both primary and established cultures of Drosophila melanogaster cells.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Rosalind Hale et al.
eLife, 4 (2015-02-25)
To understand how long-range patterning gradients are interpreted at the cellular level, we investigate how a gradient of expression of the Four-jointed kinase specifies planar polarised distributions of the cadherins Fat and Dachsous in the Drosophila wing. We use computational
Shuhei Kimura
FEBS letters, 587(7), 922-929 (2013-03-05)
Spermiogenesis is a dynamic process leading to alterations in cell morphology. In spermiogenesis, the roles of the histone chaperones are largely unknown. Here, I report the unexpected roles of two Nap family proteins, CG5017/Hanabi and nucleosome assembly protein 1 (Nap1)
Remigio Picone et al.
PLoS biology, 8(11), e1000542-e1000542 (2010-11-26)
Because physical form and function are intimately linked, mechanisms that maintain cell shape and size within strict limits are likely to be important for a wide variety of biological processes. However, while intrinsic controls have been found to contribute to
Giri Raj Dahal et al.
Development (Cambridge, England), 144(15), 2771-2783 (2017-07-08)
Loss of embryonic ion channel function leads to morphological defects, but the underlying reason for these defects remains elusive. Here, we show that inwardly rectifying potassium (Irk) channels regulate release of the
Arthur Luhur et al.
G3 (Bethesda, Md.), 10(12), 4541-4551 (2020-10-09)
Successful Drosophila cell culture relies on media containing xenogenic components such as fetal bovine serum to support continuous cell proliferation. Here, we report a serum-free culture condition that supports the growth and proliferation of Drosophila S2R+ and Kc167 cell lines.
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