R3757
Reactive Yellow 3−Agarose
saline suspension
Synonym(s):
C.I.13245
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About This Item
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form
saline suspension
Quality Level
extent of labeling
1-5 mg per mL
matrix
cross-linked 4% beaded agarose
capacity
≥500 units/mL binding capacity (citrate synthase)
storage temp.
2-8°C
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Application
Reactive Yellow 3-agarose is used in affinity chromatography, protein chromatography and dye resins. Reactive Yellow 3-agarose has been used in immunohistochemical localization studies to show where tyramine N-(hydroxycinnamoyl)transferase (THT) polypeptides occur in opium poppy. Reactive Yellow-agarose has also been used to purify human cholesteryl ester transfer protein.
Physical form
Suspension in 0.5 M NaCl containing preservative
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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The Journal of biological chemistry, 266(20), 12971-12975 (1991-07-15)
p-Aminobenzoate, a component of the vitamin folate, is one of seven compounds derived from the aromatic precursor chorismate in Escherichia coli. Historically the gene products of pabA and pabB were assumed to be sufficient for de novo p-aminobenzoate biosynthesis. Recent
The Journal of biological chemistry, 267(22), 15978-15983 (1992-08-05)
Human erythrocytes rapidly convert vitamin B6 to pyridoxal-P and contain soluble phosphatase activity which dephosphorylates pyridoxal-P at a pH optimum of 6-6.5. This phosphatase was purified 51,000-fold with a yield of 39% by ammonium sulfate precipitation and chromatography on DEAE-Sepharose
Archives of biochemistry and biophysics, 298(2), 612-619 (1992-11-01)
A mannitol:mannose 1-oxidoreductase was isolated from celeriac (Apium graveolens var. rapaceum) root tips by fractionation with (NH4)2SO4, followed by chromatography on a Fractogel DEAE column and then concentration with (NH4)2SO4. This newly discovered mannitol dehydrogenase catalyzes the NAD-dependent oxidation of
Purification of hydroxycinnamoyl-CoA:tyramine hydroxycinnamoyltransferase from cell-suspension cultures of <I>Solanum tuberosum</I> L. cv. Datura.
Planta, 199(1), 166-168 (1996)
Planta, 203(3), 349-361 (1997-01-01)
Uridine 5'-diphosphoglucose:betanidin 5-O- and 6-O-glucosyltransferases (5-GT and 6-GT; EC 2.4.1) catalyze the regiospecific formation of betanin (betanidin 5-O-beta-glucoside) and gomphrenin I (betanidin 6-O-beta-glucoside), respectively. Both enzymes were purified to near homogeneity from cell-suspension cultures of Dorotheanthus bellidiformis, the 5-GT by
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