Skip to Content
Merck
All Photos(5)

Key Documents

View All Documentation

P3375

Sigma-Aldrich

Papain from papaya latex

crude powder, 1.5-10 units/mg solid

Synonym(s):

Papainase

Sign Into View Organizational & Contract Pricing
All Photos(5)

About This Item

CAS Number:
9001-73-4
Enzyme Commission number:
3.4.22.2 (BRENDA, IUBMB)
EC Number:
232-627-2
MDL number:
MFCD00131791
UNSPSC Code:
12352204
eCl@ss:
42030311
NACRES:
NA.54

form

crude powder

Quality Level

200

specific activity

1.5-10 units/mg solid

mol wt

23.4 kDa

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Application

Papain from papaya latex has been used:
  • in the enzyme cocktail to evaluate the efficacy of B complex vitamers liberation from nutritional yeast powder
  • in the enzyme mixture for the digestion of bovine milk samples for the analysis of three vitamin B1 molecules
  • as a biocatalyst for the hydrolysis of (R,S)-naproxen 2,2,2-trifluoroethyl ester in water-saturated solvents

Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P3375, papain crude powder, has been used to digest fresh cartilage of dogsfish in order to isolate chondroitin sulfate.
Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.

Biochem/physiol Actions

Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.

Quality

Not standardized with lactose or other adulterants.

Unit Definition

One unit will hydrolyze one micromole of N-alpha-benzoyl-l-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 deg C.

Other Notes

View more information on papain at www.sigma-aldrich.com/enzymeexplorer.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Precautionary Statements

P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Comparison of different extraction methods for simultaneous determination of B complex vitamins in nutritional yeast using LC/MS-TOF and stable isotope dilution assay
Halvin K, et al.
Analytical and Bioanalytical Chemistry, 405(4), 1213-1222 (2013)
Determination of the native forms of vitamin B1 in bovine milk using a fast and simplified UHPLC method
Schmidt A, et al.
Food Chemistry, 229, 452-457 (2017)
Carica papaya lipase: a novel biocatalyst for the enantioselective hydrolysis of (R, S)-naproxen 2, 2, 2-trifluoroethyl ester
Chen CC and Tsai SW
Enzyme and Microbial Technology, 36(1), 127-132 (2005)
Changjiang Fan et al.
Macromolecular bioscience, 15(4), 535-545 (2015-01-06)
One bottleneck in tissue regeneration with hydrogel scaffolds is the limited understanding of the crucial factors for controlling hydrogel's physical microenvironments to regulate cell fate. Here, the effects of permeability and living space of hydrogels on encapsulated cells' behavior were
Tarik Bozoglu et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 9(7), e2103867-e2103867 (2022-01-14)
Adeno-associated viruses (AAVs) are frequently used for gene transfer and gene editing in vivo, except for endothelial cells, which are remarkably resistant to unmodified AAV-transduction. AAVs are retargeted here toward endothelial cells by coating with second-generation polyamidoamine dendrimers (G2) linked
View All Related Papers

Articles

Papain, Cysteine Protease, Properties & Products

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

Papain, Cysteine Protease, Properties & Products

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

Papain, Cysteine Protease, Properties & Products

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

Papain, Cysteine Protease, Properties & Products

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service