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N6635

Sigma-Aldrich

Nutrient Mixture F-10 Ham

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Ham’s F-10

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About This Item

MDL number:
MFCD00217607
UNSPSC Code:
12352207
NACRES:
NA.75

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
NaHCO3: no
sodium pyruvate: 0.11 g/L
L-glutamine: yes
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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General description

Nutrient Mixture F-10 Ham supports the clonal growth of a wide variety of cells like clones of Chinese hamster ovary [CHO] cells and a clone of HeLa and mouse L-cells. Ham′s F-10 has been developed to support the growth of human diploid cells, white blood cells for chromosomal analysis, and primary explants of rat, rabbit, and chicken tissues.

Application

Nutrient Mixture F-10 Ham has been used:

  • for the in vitro tissue culture from rabbit
  • to culture satellite cells from fish white muscle of trout
  • for culturing neonatal porcine islets (NPIs)

Quantity

Formulated to contain 9.8 grams of powder per liter of medium.

Reconstitution

Supplement with 1.2 g/L sodium bicarbonate.

also commonly purchased with this product

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Description
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supplement

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M A Silvestre et al.
Theriogenology, 58(1), 69-76 (2002-08-17)
Vitrification (3.58 M EG and 2.82 M DMSO in PBS with 20% FCS) and rapid-freezing (0.25 M sucrose, 2.25 M EG, and 2.25 M DMSO in PBS with 20% FCS) procedures were assayed to cryopreserve rabbit tissue samples from 12-day
Amirhosein Hasani et al.
Life sciences, 254, 117767-117767 (2020-05-15)
Heat stress shock affects the generation of free radicals and can have a harmful effect on spermatogenesis. Photobiomodulation (PBM) is very effective in andrology for treating male infertility. This research aimed at the evaluation of the impacts of PBM on
Fernanda de Mello et al.
General and comparative endocrinology, 210, 23-29 (2014-12-03)
Members of the TGF-β superfamily are involved in numerous cell functions; however, except for myostatin, their roles in the regulation of muscle growth in fish are completely unknown. We measured tgf-β1, tgf-β2, tgf-β3, inhibin βA (inh) and follistatin (fst) gene
Amirreza Khosravi et al.
Acta histochemica, 122(8), 151632-151632 (2020-11-01)
Testicular hyperthermia can have negative effects on male fertility. Despite reported therapeutic benefits of curcumin, several factors often limit its application such as low water solubility and instable structure. Curcumin-loaded superparamagnetic iron oxide nanoparticles (SPIONs) were designed to solve its
Azar Afshar et al.
Reproductive sciences (Thousand Oaks, Calif.), 28(2), 371-380 (2020-08-12)
Spermatogenesis process is sensitive to heat stress because the testicular temperature is 2 to 4 °C lower than the core body temperature. The current study aimed to investigate the effects of iron oxide nanoparticles containing curcumin on spermatogenesis in mice induced
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Nutrient Mixture F-10 Ham Formulation

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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