I5154
IgG1, Kappa from human myeloma plasma
purified immunoglobulin, >95% (SDS-PAGE)
Synonym(s):
Human IgG1-κ
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About This Item
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biological source
human
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
Assay
>95% (SDS-PAGE)
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
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General description
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG1 is the most abundant of the IgG subclass
Human myeloma IgG1, κ is purified from human plasma by fractionation, ion-exchange, and affinity chromatography procedures.
Human myeloma IgG1, κ is purified from human plasma by fractionation, ion-exchange, and affinity chromatography procedures.
Application
The purified IgG1, κ may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays. Human myeloma IgG1, κ was injected into mice (500 μg) to assess the T cell and B cell responses and also used for flow cytometry in CHO cells.
Physical form
Solution in 0.02 M Tris buffered saline, pH 8.0.
Analysis Note
Identity is determined by immunoelectrophoresis and indirect ELISA.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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