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I1010

Sigma-Aldrich

IgA from human colostrum

reagent grade, ≥95%, essentially salt-free, lyophilized powder

Synonym(s):

Human IgA

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

conjugate

unconjugated

Quality Level

grade

reagent grade

Assay

≥95%

form

essentially salt-free, lyophilized powder

storage temp.

2-8°C

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General description

IgA antibody is secretory antibody and is present abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears, colostrum and saliva. Thus secretory IgA contributes to the humoral defense mechanism against the pathogens on mucosal surfaces
Human IgA is purified from pooled normal human colostrum by fractionation and ion-exchange fractionation.

Application

Purified IgA may be used as a reference antigen, standard, blocking agent or coating protein in a variety of immunoassays including ELISA, dot immunobinding, immunoblotting, immunodiffusion and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solidphase immunoadsorbents. IgA from human colostrum was used as a standard in ELISA (1 mg/ml) and gel electrophoresis.

Reconstitution

Reconstitute with buffered saline (150 mM sodium chloride).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Susanna R Bidgood et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(37), 13463-13468 (2014-08-30)
IgA is the most prevalent antibody type on mucosal surfaces and the second most prevalent antibody in circulation, yet its role in immune defense is not fully understood. Here we show that IgA is carried inside cells during virus infection
Hannah Karen Mina Labayo et al.
EClinicalMedicine, 27, 100561-100561 (2020-10-13)
Norovirus (NV) causes acute gastroenteritis in infants. Humoral and fecal immunoglobulin A (IgA) responses have been correlated with protection against NV; however, the role of breast milk IgA against NV infection and associated diarrhea is still unknown. This study aimed
E Knop et al.
Investigative ophthalmology & visual science, 42(3), 566-574 (2001-02-27)
Mucosa-associated lymphoid tissue (MALT) specifically protects mucosal surfaces. In a previous study of the human conjunctiva, evidence was also found for the presence of MALT in the lacrimal sac. The present study, therefore, aims to investigate its morphology and topographical
P Kosuwon et al.
Beneficial microbes, 9(4), 541-552 (2018-04-11)
Little is known about the impact of nutrition on toddler gut microbiota. The plasticity of the toddler gut microbiota indicates that nutritional modulation beyond infancy could potentially impact its maturation. The objective of this study was to determine the effect
Erick Sánchez-Salguero et al.
Maternal health, neonatology and perinatology, 5, 9-9 (2019-06-18)
Colostrum is the primary source of maternal immunoglobulin A (IgA) for the newborn. IgA participates in protection and regulation mechanisms of the immune response at the neonate's mucosa. Several studies have evaluated infectious diseases and vaccine protocols effects during pregnancy

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