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C3041

Sigma-Aldrich

Carbonate-Bicarbonate Buffer

capsule

Synonym(s):

Carbonate Buffer Solution

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

form

capsule

Quality Level

200

pH

9.3-9.9

solubility

water, high purity: 1 capsule/100 mL

application(s)

diagnostic assay manufacturing

General description

Bicarbonate buffer mostly have a pH close to 7.4. It is the major small intestine buffer.

Application

Carbonate-Bicarbonate Buffer has been used for suspending the lyophilized human plasma sample for microtiter plate biofilm assay and flow cell biofilm assay. It has also been used as a coating buffer for microtiter plates prior to enzyme-linked immunosorbent assay(ELISA).
Prepared buffer is suitable for use in protein coating procedures on multiwell plates or plastic tubes.

Reconstitution

Empty the contents of one capsule in 100 mL of deionized water and dissolve. The contents of one capsule yields 100 ml of 0.05 M carbonate-bicarbonate buffer, pH 9.6 at 25 °C.

Other Notes

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Ashfaq Ghumra et al.
PloS one, 6(1), e16414-e16414 (2011-02-10)
Rosetting is a Plasmodium falciparum virulence factor implicated in the pathogenesis of life-threatening malaria. Rosetting occurs when parasite-derived P. falciparum Erythrocyte Membrane Protein One (PfEMP1) on the surface of infected erythrocytes binds to human receptors on uninfected erythrocytes. PfEMP1 is
Investigation of biofilm formation in clinical isolates of Staphylococcus aureus
Cassat JE, et al.
Methods in Molecular Biology, 391(1), 127-144 (2007)
Yuki Noguchi et al.
Scientific reports, 10(1), 16268-16268 (2020-10-03)
In vivo pharmacokinetics (PK) studies using mice and monkeys are the main approaches for evaluating and predicting the PK of antibodies, and there is a strong demand for methods that do not require animal experiments. In this work, we focused
Mike Burrows et al.
BMC complementary and alternative medicine, 9, 49-49 (2009-12-02)
For almost 2000 years it has been recognized that aqueous extracts from pine cones possess medicinal properties beneficial for the treatment of a broad variety of diseases and conditions. In this report, the ability of an orally administered poly phenylpropanoid-polysaccharide
Evolution of a physiological pH 6.8 bicarbonate buffer system: application to the dissolution testing of enteric coated products
Liu F, et al.
European Journal of Pharmaceutics and Biopharmaceutics, 78(1), 151-157 (2011)
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Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

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