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Key Documents

C0528

Sigma-Aldrich

Casein fluorescein isothiocyanate from bovine milk

Type III, essentially salt-free, lyophilized powder

Synonym(s):

FITC-casein

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About This Item

MDL number:
MFCD00130743
UNSPSC Code:
12352204
NACRES:
NA.32

type

Type III

Quality Level

200

form

essentially salt-free, lyophilized powder

extent of labeling

≥50 μg FITC per mg solid

solubility

water: 5 mg/mL, clear to hazy

storage temp.

2-8°C

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Application

Casein fluorescein isothiocyanate from bovine milk has been used:
  • in accessing zona pellucida (ZP) and perivitelline space (PVS) to evaluate changes induced by protease treatment
  • to make a reference line in immunochromatographic devices
  • in running gel for the documentation of proteinases

highly sensitive protease substrate

Biochem/physiol Actions

Casein fluorescein isothiocyanate is mainly used as a substrate in estimating the protease activity of psychrotrophic bacteria. It is also used as a substrate for determining the activity of bacteria in milk.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The supplementation of culture medium with protease improves the hatching rate of mouse embryos
Lee DR, et al.
Human Reproduction, 12(11), 2493-2498 (1997)
Casein, fluorescein isothiocyanate as a substrate for measuring protease production by psychrotrophic bacteria
Christen GL and Senica M
Journal of Food Protection, 50(9), 744-749 (1987)
Immunochromatographic assay for simple and rapid detection of Satsuma dwarf virus and related viruses using monoclonal antibodies
Kusano N, et al.
Journal of General Plant Pathology, 73(1), 66-71 (2007)
Tomasz Chamera et al.
Journal of molecular biology, 431(11), 2180-2196 (2019-04-27)
Hsp104 is a yeast chaperone that rescues misfolded proteins from aggregates associated with proteotoxic stress and aging. Hsp104 consists of N-terminal domain, regulatory M-domain and two ATPase domains, assembled into a spiral-shaped hexamer. Protein disaggregation involves polypeptide extraction from an
Yong Tang et al.
PLoS biology, 17(2), e3000136-e3000136 (2019-02-07)
Endosymbiosis has driven major molecular and cellular innovations. Plasmodium spp. parasites that cause malaria contain an essential, non-photosynthetic plastid-the apicoplast-which originated from a secondary (eukaryote-eukaryote) endosymbiosis. To discover organellar pathways with evolutionary and biomedical significance, we performed a mutagenesis screen
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