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Key Documents

B0556

Sigma-Aldrich

Anti-B23 antibody, Mouse monoclonal

clone FC82291, purified from hybridoma cell culture

Synonym(s):

Anti-NPM, Anti-Nucleophosmin

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About This Item

MDL number:
MFCD03457543
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

FC82291, monoclonal

form

buffered aqueous solution

mol wt

antigen 37 kDa

species reactivity

rat, kangaroo rat, canine, bovine, human, mouse, monkey, hamster

packaging

antibody small pack of 25 μL

concentration

~0.5 mg/mL

technique(s)

immunocytochemistry: suitable using 2% formaldehyde-acetone or 10% formalin/methanol-1% NP-40
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.2-0.4 μg/mL using a whole extract of 3T3 (mouse fibroblasts) cells

isotype

IgG1

UniProt accession no.

P06748

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NPM1(4869)
mouse ... Npm1(18148)
rat ... Npm1(25498)

General description

B23 (also known as NPM, nucleophosmin, numatrin or NO38) is a phosphoprotein, widely expressed in all cell types, and localized in granular regions of the nucleolus associated with pre-ribosomal particles. This protein was found to be involved in several nuclear functions such as assembly and/or intranuclear transport of preribosomal particles, cytoplasmic/nuclear trafficking, regulation of DNA polymerase A activity and centrosome duplication. Phosphorylation of B23 is mediated by a cyclin complex, CDK2/cyclin E.
Phosphorylation causes the dissociation of B23 from the centrosome and enables the duplication of the centrosome during cell mitosis. B23 was also found to be involved in pro-myelocytic leukemia in which the gene for B23 fuses with retinoic acid receptor a (RARa). As a consequence, a fusion protein (60-7 0 kDa) is produced containing parts of the two genes.

Specificity

It detects both the phosphorylated and the unphosphorylated B23 molecule. The epitope recognized by the antibody lies within the 68 amino acids at the C-terminus of B23.

Immunogen

rat B23 (nucleophosmin, NPM, numatrin).

Application

The antibody may be used for ELISA, competitive ELISA, immunoblotting (37 kDa), immunoprecipitation, immunocytochemistry (2% formaldehyde-acetone 1,2,5 or 10% formalin/methanol-1% NP-40 and microinjection (blocks the initiation of centrosome duplication) and immunohistochemistry. Reactivity has been observed with human, monkey, bovine, dog, hamster (weak), rat,kangaroo rat, and mouse B23.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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