A4781
Asialofetuin from fetal calf serum
Type I (Sigma designation)
Synonym(s):
Asialofetuin
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About This Item
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biological source
bovine (calf) serum
Quality Level
type
Type I (Sigma designation)
form
powder
impurities
salt, essentially free
≤0.5% N-acetylneuraminic acid
solubility
0.85% sodium chloride: soluble 1 mg/mL
storage temp.
2-8°C
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General description
Asialofetuin is a glycoprotein with three asparagine-linked triantennary complex carbohydrate chains and terminal N-acetylgalactosamine residues.
Application
Asialofetuin from fetal calf serum has been used:
- to quantitate the plant Ricin toxin′s B subunit (RTB) lectin activity in β-phaseolin signal peptide (P)–proinsulin gene (INS)–RTB plants by enzyme-linked immunosorbent assay (ELISA)
- quantitate VP7:RTB fusion protein in transformed potato tissues by ELISA
- as a glycoprotein substrate to measure the receptor-binding activity of recombinant RTB and NSP490–RTB fusion proteins
- to study the nature of the interaction between ferritin and the placenta
Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy.
Biochem/physiol Actions
Asialofetuin exhibits affinity to asialoglycoprotein receptor (ASGP-R) on hepatocytes and uses the receptor to enter the cell. This property allows asialofetuin to be used as a ligand to deliver drugs to hepatocytes and as a competitive inhibitor to ASGP-R.
Preparation Note
Prepared by a modification of Spiro, R.G., J. Biol. Chem., 235, 2860 (1960).
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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The purpose of this study is to evaluate in vitro gene delivery mediated by asialofetuin-appended cationic liposomes (AF-liposomes) associating cyclodextrins (CyD/AF-liposomes) as a hepatocyte-selective nonviral vector. Of various CyDs, AF-liposomes associated with plasmid DNA (pDNA) and γ-cyclodextrin (γ-CyD) (pDNA/γ-CyD/AF-liposomes) showed
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