The solution is 50% glycerol with 75mM NaCL, in addition to the presence of 50 mg of Apoferritin per vial. However, a 50% glycerol solution is typically expected to have a density of 1.26 g/ml.
Pricing and availability is not currently available.
Recommended Products
form
glycerol solution (50% glycerol and 0.075 M NaCl)
Quality Level
packaging
vial of 50 mg
storage temp.
−20°C
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Application
Apoferritin is a gel filtration molecular weight marker that can be used in gel filtration chromatography and protein chromatography. Apoferritin has the ability to demetallate hemin and to metallate protoporphyrin IX.
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Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Noëlle Carette et al.
Journal of inorganic biochemistry, 100(8), 1426-1435 (2006-06-20)
Earlier crystallographic and spectroscopic studies had shown that horse spleen apoferritin was capable of removing the metal ion from hemin (Fe(III)-protoporphyrin IX) [G. Précigoux, J. Yariv, B. Gallois, A. Dautant, C. Courseille, B. Langlois d'Estaintot, Acta Cryst. D50 (1994) 739-743;
Xindong Wang et al.
Biosensors & bioelectronics, 47, 171-177 (2013-04-11)
A novel sandwich electrochemical immunoassay was developed for ultrasensitive detection of avian leukosis virus subgroup J (ALVs-J) using graphene quantum dots (GQDs) and apoferritin-encapsulated Cu (Cu-apoferritin) nanoparticles for signal amplification. GQDs were used both for the conjugation of primary ALVs-J
Venkata P Dandey et al.
Journal of structural biology, 202(2), 161-169 (2018-01-26)
We present an update describing new features and applications of Spotiton, a novel instrument for vitrifying samples for cryoEM. We have used Spotiton to prepare several test specimens that can be reconstructed using routine single particle analysis to ∼3 Å resolution
David P Klebl et al.
Acta crystallographica. Section D, Structural biology, 76(Pt 4), 340-349 (2020-04-08)
Despite the great strides made in the field of single-particle cryogenic electron microscopy (cryo-EM) in microscope design, direct electron detectors and new processing suites, the area of sample preparation is still far from ideal. Traditionally, sample preparation involves blotting, which
Richard Henderson et al.
Microscopy (Oxford, England), 62(1), 43-50 (2013-01-08)
Theoretical considerations together with simulations of single-particle electron cryomicroscopy images of biological assemblies in ice demonstrate that atomic structures should be obtainable from images of a few thousand asymmetric units, provided the molecular weight of the whole assembly being studied
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What is the density of A3660-1vl (Apoferritin from equine spleen)?
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What is the purity percentage of A3660?
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Unfortunately, this product is not tested for purity.
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Can Product A3660, Apoferritin from equine spleen, be sterilized?
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Yes. First the glycerol will need to be removed (typically by dialysis). Then a solution of apoferritin in 0.15M sodium chloride can be filtered through a 0.2 micron filter, as is done for Product No. A3641.
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What is the molecular weight of Product A3660, Apoferritin from equine spleen?
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The approximate molecular weight of this product is 443,000.
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What is the concentration of Product A3660, Apoferritin from equine spleen?
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The concentration is roughly 25 mg/mL, according to the technical bulletin.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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