75259

Tris(2-carboxyethyl)phosphine hydrochloride

BioUltra, ≥98% (NMR)

• Synonym(s): TCEP
• Empirical Formula (Hill Notation): C₉H₁₅O₆P · HCl
• CAS Number: 51805-45-9
• Molecular Weight: 286.65
• Beilstein: 3724376
• MDL number: MFCD00145469
• UNSPSC Code: 12352128
• PubChem Substance ID: 329766294
• NACRES: NA.25

Properties

• product line: BioUltra
• Quality Level: 300
• Assay: ≥98% (NMR)
• form: crystals
• reaction suitability: reagent type: reductant
• loss: ≤1.0% loss on drying
• pH: 1.2-1.5
• solubility: H₂O: 1 M, clear, colorless (no residue)
• anion traces: sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤5 mg/kg; As: ≤0.05 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 0.1 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.05; λ: 280 nm A_max: 0.03
• storage temp.: 2-8°C
• SMILES string: Cl[H].OC(=O)CCP(CCC(O)=O)CCC(O)=O
• InChI: 1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H
• InChI key: PBVAJRFEEOIAGW-UHFFFAOYSA-N

Description

General description

Tris(2-carboxyethyl)-phosphine hydrochloride (TCEP) is a strong reducing agent that is stable in an aqueous solution. It readily and stoichiometrically reduces disulfides with high specificity. TCEP exhibits reducing activity at pH 1.5 to pH 9.0 and reduces dehydroascorbic acid (DHA) at pH 4.

Application

Tris(2-carboxyethyl)phosphine hydrochloride has been used:

• as a component of IRE1 dialysis buffer, RNA cleavage buffer, and in in vitro cleavage assay of in vitro transcribed RNA
• in reducing sulfhydryl groups to obtain total thiol fraction
• to reduce protein samples

Water soluble reagent, used for selective reduction of disulfides. More stable than DTT and useful in mass spectrometry applications.

Safety Information

• Pictograms: GHS05
• Signal Word: Danger
• Hazard Statements: H314
• Precautionary Statements: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Hazard Classifications: Eye Dam. 1 - Skin Corr. 1B
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 1