AP162P
Rabbit Anti-Chicken IgG Antibody, HRP conjugate
0.8 mg/mL, Chemicon®
Synonym(s):
IgY
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46
Recommended Products
biological source
rabbit
Quality Level
conjugate
peroxidase conjugate
antibody form
F(ab′)2 fragment of affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
chicken
manufacturer/tradename
Chemicon®
concentration
0.8 mg/mL
technique(s)
ELISA: suitable
western blot: suitable
shipped in
wet ice
target post-translational modification
unmodified
General description
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Specificity
Chicken IgY (IgG (H+L))
ANTIBODY SPEIFICITY:
Based on immunoelectrophoresis, the antibody reacts with the heavy chainjs of Chicken IgY and with light chains common to most chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins, but antibodies may cross-react with immunoglobulins from other species.
ANTIBODY SPEIFICITY:
Based on immunoelectrophoresis, the antibody reacts with the heavy chainjs of Chicken IgY and with light chains common to most chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins, but antibodies may cross-react with immunoglobulins from other species.
Application
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Whole Immunoglobulin Secondary Antibodies
Suggested dilution for most applications: 1:500-1:200,000 for most applications. Optimal working dilutions must be determined by the end user.
This Rabbit anti-Chicken IgG Antibody, HRP conjugate is validated for use in ELISA, WB for the detection of Chicken IgG.
Physical form
Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA. Contains no preservative.
Storage and Stability
Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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