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Key Documents

L040030

Sigma-Aldrich

Cap A

configured for ABI

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.21

type

for DNA synthesis

Quality Level

200

product line

Proligo Reagents

form

liquid

shelf life

365 days

concentration

97.0-119.0 g/L (Acetic Anhydride content, titration)

color

clear colorless, to brownish

compatibility

configured for ABI

storage temp.

2-8°C

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General description

Cap A solution contains acetic anhydrideanhydride, lutidine and tetrahydrofuran (THF). It is used after the phosphoramidite reaction/coupling step. The solution is configured for ABI.

Biochem/physiol Actions

Cap A solution aborts chains bearing unreacted 5′ hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′ hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

Preparation Note

Tetrahydrofurane / Lutidine / Acetic Anhydride 8/1/1 (v/v/v)

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Supplementary Hazards

EUH019

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

6.1 °F

Flash Point(C)

-14.4 °C

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number
HMBK2893
HMBK2474
HMBK4035
HMBK4033
HMBK2121

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