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Key Documents

G006143

HPLC Analysis of Proteins and Peptides on Proteomix® WCX, Comparison of Different Particle Sizes

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Proteomix WCX NP3, 5 cm x 4.6 mm I.D., 3 μm particles (Z777185)
column: Proteomix WCX-NP1.7, 5 cm x 4.6 mm I.D., 1.7 μm particles (Z777180)
column: Proteomix WCX-NP10, 5 cm x 4.6 mm, 10 μm particles (Z777169)
column: Proteomix WCX-NP5, 5 cm x 4.6 mm x 5 μm particles (Z777193)
mobile phase: (A) 20 mM sodium phosphate, pH 6.5, (B) A + 1.0 M NaCl
gradient: 0 to 100% B in 25 min, 15 min prewash
flow rate: 0.5 mL/min
column temp.: ambient
detector: UV, 214 nm
injection: 5 μL
sample: Ribonuclease A, Cytochrome C and Lysozyme (1 mg/mL) min

suitability

application for HPLC

application(s)

life science and biopharma
pharmaceutical (small molecule)

Legal Information

Proteomix is a registered trademark of Sepax Technologies

Analyte

Description
    Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
    Cytochrome c from chicken heart, ≥95% based on Mol. Wt. 12,222 basis
    Lysozyme from chicken egg white, powder or granules, ≥90 %, ≥39,000 units/mg protein

analytical column

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