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58550AST

CHIRALPAK® CBH (5 μm) HPLC Columns

L × I.D. 10 cm × 4 mm, HPLC Column

Synonym(s):

CBH Chiral HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

CHIRALPAK® CBH HPLC Column, 5 μm particle size, L × I.D. 10 cm × 4 mm

material

stainless steel column

Quality Level

product line

CHIRALPAK®

packaging

pkg of 1 ea

manufacturer/tradename

CHIRALPAK®

parameter

137 bar pressure (2000 psi)
20-30 °C temperature
40 °C max. temp.

technique(s)

HPLC: suitable

L × I.D.

10 cm × 4 mm

matrix

fully porous particle

matrix active group

cellobiohydrolase phase

particle size

5 μm

operating pH

2-8

separation technique

chiral

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General description

Hermansson described the use of natural proteins immobilized onto a silica support for chiral separations in 1983 (1). Proteins contain a large number of chiral centers of one configuration, and many other sites that contribute to the general retention process. Used primarily for the separation enantiomers of basic compounds, CHIRALPAK CBH phase has cellobiohydrolase as the chiral selector immobilized on spherical 5 μm silica particles. Used in reversed-phase mode, retention and enantioselectivity is regulated by changes of pH, buffer concentration and organic modifier. The mobile phases are mixtures of phosphate or acetate buffers and organic solvents such as 2-propanol or acetonitrile. The column is preferably used for the separation of enantiomers of basic drugs, particularly compounds containing one or more nitrogen atoms along with one or more hydrogen-bonding groups (alcohol, phenol, carbonyl, amide, ether, ester, etc.).

Features:

  • Direct reversed phase resolution of chiral molecules
  • Stable with a variety of organic modifiers
  • Available in analytical and semi-preparative sizes

(1) Hermansson, J. Direct liquid chromatographic resolution of racemic drugs using a1-acid glycoprotein as the chiral stationary phase. J. Chromatogr. A, 1983, 269, 71-80.

Application


  • Simultaneous enantioselective analysis of illicit drugs in wastewater and surface water by chiral LC-MS/MS: A pilot study on a wastewater treatment plant and its receiving river.: This study utilizes the CHIRALPAK CBH HPLC Column for the enantioselective analysis of illicit drugs in environmental water samples, demonstrating its application in monitoring wastewater and surface water quality (Wang et al., 2021).

  • Enantiomeric determination of cathinones in environmental water samples by liquid chromatography-high resolution mass spectrometry.: This research highlights the application of the CHIRALPAK CBH HPLC Column in the enantiomeric separation of cathinones, providing a method for the analysis of these substances in environmental water samples using LC-HRMS (Fu et al., 2020).

  • Comparison of polysaccharide-based and protein-based chiral liquid chromatography columns for enantioseparation of drugs.: This paper compares the enantioseparation efficiency of polysaccharide-based and protein-based chiral LC columns, including the CHIRALPAK CBH HPLC Column, for the separation of various drugs, emphasizing its effectiveness and application in pharmaceutical analysis (Fu et al., 2020).

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Legal Information

CHIRALPAK is a registered trademark of Daicel Corp.

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