567502-U

Discovery^® HS F5 (3 µm) HPLC Columns

L × I.D. 10 cm × 2.1 mm, HPLC Column

• Synonym(s): Discovery Pentafluorophenyl PFP HPLC Column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: Discovery^® HS F5 HPLC Column, 3 μm particle size, L × I.D. 10 cm × 2.1 mm
• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L43
• product line: Discovery^®
• feature: endcapped
• manufacturer/tradename: Discovery^®
• packaging: 1 ea of
• extent of labeling: 12% Carbon loading
• parameter: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 10 cm × 2.1 mm
• surface area: 300 m²/g
• surface coverage: 4 μmol/m²
• impurities: <10 ppm metals
• matrix: silica gel, high purity, spherical particle platform; fully porous particle
• matrix active group: PFP (pentafluorophenyl) phase
• particle size: 3 μm
• pore size: 120 Å
• operating pH range: 2-8
• application(s): food and beverages
• separation technique: reversed phase

Description

General description

Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log P_o/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.

The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.

Application

• Optimization and validation of a bioanalytical HPLC-UV technique for simultaneous determination of underivatized phenylalanine and tyrosine in the blood for phenylketonuria diagnosis and monitoring.: This study optimizes and validates an HPLC-UV method using a Discovery^® HS F5 HPLC Column for the simultaneous determination of phenylalanine and tyrosine in blood samples, providing a robust tool for diagnosing and monitoring phenylketonuria. (Rabti et al., 2024).


• Heart-cut bidimensional achiral-chiral liquid chromatography applied to the evaluation of stereoselective metabolism, in vivo biological activity, and brain response to chiral drug candidates targeting the central nervous system.: The study uses the Discovery^® HS F5 HPLC Column in a bidimensional achiral-chiral liquid chromatography setup to evaluate the stereoselective metabolism and biological activity of chiral CNS drug candidates. (Battisti et al., 2016).


• Chromatographic separation of arsenic species with pentafluorophenyl column and application to rice.: This paper describes the separation of arsenic species in rice using a pentafluorophenyl column, highlighting the effectiveness of the Discovery^® HS F5 HPLC Column for environmental and food safety applications. (Baba et al., 2014).


• Advantages of pentafluorophenylpropyl stationary phase over conventional C18 stationary phase--application to analysis of triamcinolone acetonide.: The research compares the Discovery^® HS F5 HPLC Column to conventional C18 columns, demonstrating superior performance in the analysis of triamcinolone acetonide. (Havlíková et al., 2008).

Features and Benefits

• Unique selectivity
• Similar retention to C18 (sometimes requires stronger mobile phase)
• Excellent peak shape
• Stable, low-bleed LC-MS separations
• Scalable separations from 3 to 10μm particle sizes

Recommended products

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Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable