T1892
Synonym(s):
Direct Yellow 7
Thioflavine S, practical grade
practical grade
powder
microbe id | staining: suitable
light beige to dark beige, to Dark Brown
ethanol: water (1:1): 1 mg/mL
360-380
diagnostic assay manufacturing
hematology
histology
room temp
S1c2c(ccc(c2)C)N(C1=C3C=CC(=[N+](C)C)C=C3)C.[Cl-]
1S/C17H19N2S.ClH/c1-12-5-10-15-16(11-12)20-17(19(15)4)13-6-8-14(9-7-13)18(2)3;/h5-11H,1-4H3;1H/q+1;/p-1
JADVWWSKYZXRGX-UHFFFAOYSA-M
Warning
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Respiratory system
11 - Combustible Solids
WGK 3
Not applicable
Not applicable
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Yes. Thioflavine S is a fluorescent dye. Excitation is at 391 nm and emission is at 428 nm. The microscope companies that sell fluorescent microscopes will be able to offer the suggested filter sets for the fluorescent microscope you are using
This product is listed as a practical grade dye and is not highly purified. Quality control testing does not include testing for either the excitation or emission wavelengths.
Helpful?
How should Thioflavine S be reconstituted at 1mg/mL?
For usage, Thioflavine S is typically dissolved at a concentration of 1 gram of dye in 100 ml of water (10mg/ml). Sections are taken to water and then stained for 7 minutes in the Thioflavine S solution. Slides are rinsed in 3 changes of 80% alcohol. Subsequently, the slides are dehydrated, cleared, and mounted in fluorescent free mounting media, such as DPX. Finally, the slides are viewed with a fluorescent microscope.
Helpful?
Regarding item T1892-25G, how much time is needed to rinse the section in 80% EtOH and what is the time for the dehydration step?
When the time for rinse questions arises, it generally means the slides are rinsed in a particular solvent after the slides have been stained in the staining reagent. Protocols can and will vary for Thioflavine T. The procedure calls for deparaffinizing sections in the normal fashion. Slides are then stained in Mayers Hematoxylin for 2 minutes and washed in tap water for 3 to 5 minutes to blue the nuclei. Subsequently, the slides are stained for 3 minutes in the Thioflavine T solution. After this, the slides are rinsed in distilled water with no specified length of time. The slides are then differentiated in 1% acetic acid for 20 minutes, followed by a wash in running tap water for 2 minutes. Finally, the slides are blotted dry and mounted in an appropriate fluorescent mounting media. Other than the deparaffinization procedure, the slides are not rinsed in 80% alcohol. After the slides are blotted dry, there is no normal dehydration routine that is common to slides being mounted in a permanent mounting medium.
Helpful?
Whether Thioflavine S requires a conjugated secondary antibody or if it is the only stain to be applied to the tissue?
No, Thioflavine S is a fluorescent dye that binds to amyloid and fibrillar material in abnormal structures. Sections requiring viewing under a fluorescence microscope with filters for Thioflavine S. Slides are typically stained for 7 minutes in a 1% solution of Thioflavine S prepared in distilled water. The slides are then washed in 3 changes of 80% alcohol. Subsequently, the slides are further dehydrated, cleared, and then mounted using a fluorescent-free mounting media. After these procedures, the slides are ready for viewing with a fluorescent microscope.
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