SRP0354
Aurora Kinase A active human
recombinant, expressed in baculovirus infected Sf9 cells, ≥90% (SDS-PAGE)
Synonym(s):
ARK1, AURA, BTAK, STK6
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About This Item
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biological source
human
recombinant
expressed in baculovirus infected Sf9 cells
Assay
≥90% (SDS-PAGE)
form
aqueous solution
mol wt
50 kDa
packaging
pkg of 10 μg
technique(s)
activity assay: suitable
inhibition assay: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... AURKA(6790)
General description
AURA/AURKA (aurora kinase A) is mapped to human chromosome 20q13.2. It is also termed as STK15 (serine/threonine kinase 15). This gene codes for a protein of 403 amino acids with regulatory and catalytic domains. AURA is overexpressed in several malignancies like invasive carcinomas of ovary, head, neck and breast.
Human Aurora kinase A (GenBank Accession No. NM_003600), amino acids 2- 403 with N-terminal HIS6-tag, MW=50 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
AURA/AURKA (aurora kinase A) modulates cell cycle checkpoints and plays important roles in centrosome division, cytoskeleton and cytokinesis. It sustains the integrity of chromosomes after DNA damage. The catalytic domain of AURKA possesses a serine/threonine kinase activity.
Unit Definition
One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substarte per minute at pH 7.4 and 30°C.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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A single nucleotide polymorphism in codon F31I and V57I of the AURKA gene in invasive ductal breast carcinoma in Middle East
Golmohammadi R, et al.
Medicine, 96(37) (2017)
Sunyi Lee et al.
FEBS letters, 588(17), 2844-2850 (2014-07-06)
IK is known to inhibit the expression of major histocompatibility complex (MHC) class II antigen, but other cellular functions of IK remain to be uncovered. In this study, IK depletion caused misalignment of chromosomes through an increase in Aurora A
Naoko Kunitoku et al.
Developmental cell, 5(6), 853-864 (2003-12-12)
The Aurora (Ipl1)-related kinases are universal regulators of mitosis. We now show that Aurora-A, in addition to Aurora-B, regulates kinetochore function in human cells. A two-hybrid screen identified the kinetochore component CENP-A as a protein that interacts with Aurora-A. Aurora-A
Mutsuko Ouchi et al.
The Journal of biological chemistry, 279(19), 19643-19648 (2004-03-03)
Aurora-A/BTAK/STK15 localizes to the centrosome in the G(2)-M phase, and its kinase activity regulates the G(2) to M transition of the cell cycle. Previous studies have shown that the BRCA1 breast cancer tumor suppressor also localizes to the centrosome and
Veronica Rendo et al.
Nature communications, 11(1), 1308-1308 (2020-03-13)
Cancer chemotherapy targeting frequent loss of heterozygosity events is an attractive concept, since tumor cells may lack enzymatic activities present in normal constitutional cells. To find exploitable targets, we map prevalent genetic polymorphisms to protein structures and identify 45 nsSNVs (non-synonymous
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