SRP0237

PI3 kinase (p120g) Active human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

• Synonym(s): PIK3CG, Phosphoinositide-3-kinase, catalytic, gamma, p120-PI3K
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• Assay: ≥90% (SDS-PAGE)
• form: aqueous solution
• specific activity: ≥1.4 pmol/min-μg
• mol wt: 131 kDa
• packaging: pkg of 20 μg
• concentration: >0.02 mg/mL
• NCBI accession no.: AF327656
• UniProt accession no.: P48736
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PIK3CG(5294)

Description

General description

Human p120γ ( GenBank Accession No. AF327656), full length with N-terminal His tag, MW = 131 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.