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S3402

Sigma-Aldrich

Streptavidin-R-Phycoerythrin from Streptomyces avidinii

buffered aqueous solution

Synonym(s):

Streptavidin-R-PE

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

conjugate

phycoerythrin (R-PE) conjugate

Quality Level

form

buffered aqueous solution

mol wt

~60 kDa (four identical subunits)

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5 μg/mL using human appendix or human tonsil tissue

shipped in

wet ice

storage temp.

2-8°C

General description

Streptavidin is a tetrameric protein having high affinity for biotin. Streptavidin-R-Phycoerythrin is used to stain PNA zip-code microarray to produce a fluorescent signal. The product reacts specifically with antiserum to streptavidin but not with antiserum to avidin.

Application

Streptavidin-R-Phycoerythrin along with biotinylated reagents in an avidin/biotin labeling system can be used in flow cytometry.
The streptavidin-phycoerythrin (PE) conjugate may be used in conjunction with biotinylated reagents in the avidin/biotin labeling system in flow cytometry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Prepared by conjugation to R-phycoerythrin (PE). This orange-red dye is efficiently excited at 488 nm and emits at 578 nm.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Jae Yang Song et al.
Nucleic acids research, 33(2), e19-e19 (2005-02-03)
In the present study, we exploited the superior features of peptide nucleic acids (PNAs) to develop an efficient PNA zip-code microarray for the detection of hepatocyte nuclear factor-1alpha (HNF-1alpha) mutations that cause type 3 maturity onset diabetes of the young

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