R1008
Monoclonal Anti-Rabbit IgG (γ-chain specific) antibody produced in mouse
clone RG-96, ascites fluid
Synonym(s):
Monoclonal Anti-Rabbit IgG (γ-chain specific)
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Recommended Products
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
secondary antibodies
clone
RG-96, monoclonal
contains
15 mM sodium azide
species reactivity
rabbit
should not react with
goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken
technique(s)
dot blot: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
indirect ELISA: 1:2,000
western blot: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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General description
Monoclonal Anti-Rabbit IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins (Igs) belongs immunoglobulin super-family. There are five different types of immunoglobulins. Each immunoglobulin has two heavy (H) and two light (L), held together by disulphide linkages. Each light comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region.
The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.
Specificity
Monoclonal Anti-Rabbit IgG is specific for an epitope located on the heavy (γ) chain of rabbit IgG, in an immunoblot of denatured, reduced rabbit IgG. The antibody does not react with rabbit IgA, IgM or light chains. No cross reactivity is observed with IgG from bovine, cat, chicken, dog, goat, guinea pig, horse, human, pig, rat or sheep.
Application
Monoclonal Anti-Rabbit IgG (γ-chain specific) antibody produced in mouse has been used in
- enzyme linked immunosorbent assay (ELISA)
- dot blots
- immunohistology
- western blotting
- peptide mass fingerprinting (PMF)
Biochem/physiol Actions
Digestion of IgG by papain generates fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallisable (fc). IgG antibody has enormous therapeutic potential and the Fc region is involved in the development of therapeutic antibody.
Physical form
The product is provided as ascites fluid with 15 mM sodium azide (see MSDS) as a preservative.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Jeremy K Brown et al.
The American journal of pathology, 165(1), 95-106 (2004-06-25)
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Area-Gomez E, et al.
The American Journal of Pathology, 175(5), 1810-1816 (2009)
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