PP0520

GlycoProfile^™ 2-AB Labeling Kit

• Synonym(s): 2-AB Labeling Kit
• NACRES: NA.32
• UNSPSC Code: 12352200

Properties

• Quality Segment: 200

Description

General description

The GlycoProfile^™ labeling kits are designed for efficient labeling of N-linked, O-linked, and glycosyl­phosphatidyl­inositol (GPI) anchored glycans with either 2-aminobenzamide (2-AB) or 2-aminobenzoic acid (anthranilic acid; 2-AA). These small fluorophores increase the spectral absorption of glycans, improving the detection of labeled glycans by HPAE (high-performance anion exchange chromatography), HPLC, and ESI-MS (electrospray mass spectroscopy) methods. Binding is robust and the resulting dye-glycan conjugates are stable, with no degradation during post-labeling analysis.

Application

While 2-AB is slightly less sensitive than 2-AA, it is suitable for downstream glycan analysis by HPAE, HPLC and ESI-MS. Although suitable for the same applications, 2-AA is better suited for SDS-PAGE.

GlycoProfile^™ 2-AB Labeling Kit has been used for the labelling of N-linked glycans from human IgG, Bovine fetuin, and human epididymis protein 4. It has also been used for labelling free oligosaccharides from mouse spleen.

Legal Information

Labeling of glycans with 2-AB is covered under US Patent No. 5,747,347 and its foreign equivalents.

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

• 2-AB Labeling Reagent 2 x 5
• Dimethyl sulfoxide (DMSO) 2 x 350
• Acetic acid, glacial 2 x 200
• Reductant (sodium cyanoborohydride) 2 x 6

Safety Information

• Pictograms: GHS02,GHS06,GHS05,GHS09
• Signal Word: Danger
• supp_hazards: EUH032
• Storage Class: 3 - Flammable liquids
• Hazard Codes: H226,H300 + H310 + H330,H314,H410
• Precautionary Statements: P210 - P280 - P301 + P310 + P330 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 1 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A