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P4244

Sigma-Aldrich

Phosphate buffered saline

liquid, pH 7.2 (25 °C), autoclaved

Synonym(s):

PBS

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About This Item

MDL number:
UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25

product name

Phosphate buffered saline, Autoclaved, pH 7.2 (25 °C)

description

Sodium chloride, 150 mM, and sodium phosphate, 150 mM

form

liquid

pH

7.2 (25 °C)

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

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General description

Phosphate buffered saline (PBS) is an isotonic standard buffer solution. PBS imitates the pH, ion concentrations, and osmolarity of the human body. It serves as an ideal buffer for maintaining the osmolarity and physiological pH for biomedical dilutions in cell culture and protein chemistry. PBS is used for standard enzymatic tests with active metal-organic framework (MOF) biocomposites. PBS is considered non-toxic to cells.

Application

Phosphate buffered saline has been used:
  • for washing bacterial cells after harvesting by centrifugation
  • for washing the peritoneal cavity for isolation of peritoneal macrophages
  • as a control and to dilute phytohemagglutinin (PHA) to prepare subcutaneous injections and study the immune response in birds.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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