Key Documents

Orange G

Name: Orange G
Brand: SIGMA

for NA electrophoresis

Synonym(s):

1-Phenylazo-2-naphthol-6,8-disulfonic acid disodium salt, 7-Hydroxy-8-phenylazo-1,3-naphthalenedisulfonic acid disodium salt, Acid Orange 10, Wool Orange 2G

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About This Item

Empirical Formula (Hill Notation):

C₁₆H₁₀N₂Na₂O₇S₂

CAS Number:

1936-15-8

Molecular Weight:

452.37

EC Number:

217-705-6

UNSPSC Code:

12171500

NACRES:

NA.52

PubChem Substance ID:

57654483

MDL number:

MFCD00012457

Beilstein/REAXYS Number:

4120705

Colour Index Number:

16230

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Properties

grade

Molecular Biology

Quality Segment

200

form

powder

technique(s)

nucleic acid detection: suitable

solubility

water: 1 mg/mL, clear, orange to red

suitability

suitable for gel electrophoresis

storage temp.

room temp

SMILES string

[Na+].[Na+].Oc1ccc2cc(cc(c2c1\N=N\c3ccccc3)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C16H12N2O7S2.2Na/c19-13-7-6-10-8-12(26(20,21)22)9-14(27(23,24)25)15(10)16(13)18-17-11-4-2-1-3-5-11;;/h1-9,19H,(H,20,21,22)(H,23,24,25);;/q;2*+1/p-2/b18-17+;;

InChI key

HSXUHWZMNJHFRV-QIKYXUGXSA-L

Description

General description

Orange G is an azo dye that is acidic in nature. It is widely used as a tracking dye for nucleic acid gel electrophoresis to track DNA front in agarose gels. Typically runs at 50bp in up to 2.5% agarose.

Application

Orange G has been used as a DNA gel loading dye.

Orange G is a tracking dye used in nucleic acid electrophoresis to track DNA front in agarose gels. Also used in SDS-capillary electrophoresis as a standard. Typically runs at 50bp in up to 2.5% agarose.

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This Item	861286	O7252	A6014
Sigma-Aldrich O3756 Orange G	Sigma-Aldrich 861286 Orange G	Sigma-Aldrich O7252 Orange G	Sigma-Aldrich A6014 Acridine Orange hemi(zinc chloride) salt
description for NA electrophoresis	description certified by the BSC, Dye content 80 %	description certified by the BSC	description For nucleic acid staining in cells or gels
suitability suitable for gel electrophoresis	suitability -	suitability -	suitability suitable for flow cytometry, suitable for microscopy
technique(s) nucleic acid detection: suitable	technique(s) microbe id \| staining: suitable	technique(s) microbe id \| staining: suitable	technique(s) nucleic acid detection: suitable
grade Molecular Biology	grade -	grade -	grade -
solubility water: 1 mg/mL, clear, orange to red	solubility H₂O: 1 mg/mL	solubility water: 1 mg/mL, clear	solubility 4 mg/mL (2-methoxyethanol (EGME)), ethanol: 2 mg/mL, water: 6 mg/mL (Forms a clear, dark orange or amber solution at 1mg/mL.)
form powder	form powder	form powder	form powder
storage temp. room temp	storage temp. room temp	storage temp. room temp	storage temp. room temp

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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