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MAK068

Sigma-Aldrich

MPO Colorimetric Activity Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

Myeloperoxidase Colorimetric Activity Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

cancer; cardiovascular diseases; hematological disorder

storage temp.

−20°C

Gene Information

human ... MPO(4353)
mouse ... MPO(17523)
rat ... MPO(303413)

General description

Myeloperoxidase (MPO) is a heme-containing enzyme that catalyzes the hydrogen peroxidase-mediated oxidation of halide ions to hypochlorous acid. MPO is a lysosomal protein, highly expressed in neutrophils, that plays a role in the antimicrobial actions that occur as a result of neutrophil stimulation. The actions of MPO may also contribute to the initiation and pathogenesis of cardiovascular disease.

Application

MPO Colorimetric Activity Assay Kit has been used to determine the activity of myeloperoxidase in samples.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of myeloperoxidase (MPO) in biological samples such as tissue, cells, serum and white blood cells

Principle

In this assay, MPO catalyzes the formation of hypochlorous acid, which reacts with taurine to form taurine chloroamine. Taurine chloroamine reacts with the chromophore TNB, resulting in the formation of the colorless product DTNB. One unit of MPO activity is defined as the amount of enzyme that hydrolyzes the substrate and generates taurine chloramine to consume 1.0 μmole of TNB per minute at 25 °C.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H315,H319,H334

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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