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MCDB 131 Medium

Name: MCDB 131 Medium
Brand: SIGMA

With ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

131 Medium, Medium 131, Molecular, Cellular, and Development Biology 131 medium

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

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Properties

Product Name

MCDB 131 Medium, With trace elements and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

form

powder

concentration

11.6 g/L

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.11 g/L
phenol red: 0.012 g/L
L-glutamine: 1.461 g/L
HEPES: no
glucose: 1.0 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

Description

General description

MCDB-131 medium provides a defined and optimally balanced nutritional environment that selectively promotes the growth of specific cell types. MCDB-131 is designed to support clonal growth of human microvascular endothelial cells, including human umbilical vein endothelial cells, when supplemented with 0.7% FBSP, 10 ng/ml epidermal growth factor, and 1 μg/ml hydrocortisone. MCDB media is made for the low-protein or serum-free growth of specific cell types using hormones, growth factors, trace elements, or low levels of dialyzed fetal bovine serum protein (FBSP).

Application

MCDB-131 Medium has been used:

as a component of the basal medium to culture bovine retinal endothelial cells
to culture bovine lung microvascular endothelial cells
for the isolation of porcine endocranial endothelial cells and porcine aortic endothelial cells

Preparation Note

Formulated to contain 11.6 grams of powder per liter of medium.

Supplement with 1.18 g/L sodium bicarbonate.

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This Item	M6395	M6770	F8105
Sigma-Aldrich M8537 MCDB 131 Medium	Sigma-Aldrich M6395 MCDB 105 Medium	Sigma-Aldrich M6770 MCDB 201 Medium	Sigma-Aldrich F8105 MCDB 153 Medium
description With trace elements and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture	description With trace elements, L-glutamine and 25mM HEPES, powder, suitable for cell culture	description With trace elements, L-glutamine and 30 mM HEPES, powder, suitable for cell culture	description With trace elements, and 28mM HEPES, with sodium bicarbonate, without L-glutamine, liquid, suitable for cell culture
form powder	form powder	form powder	form liquid
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
components sodium pyruvate: 0.11 g/L phenol red: 0.012 g/L L-glutamine: 1.461 g/L HEPES: no glucose: 1.0 g/L (Dextro)	components phenol red: 0.001242 g/L glucose: 0.72064 g/L (Dextro) sodium pyruvate: 0.11 g/L HEPES: 5.958 g/L (25mM) L-glutamine: 0.3653 g/L	components HEPES: 7.149 g/L (30mM) glucose: 1.441 g/L (Dextro) phenol red: 0.001242 g/L sodium pyruvate: 0.055 g/L L-glutamine: 0.14615 g/L	components L-glutamine: no
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
concentration 11.6 g/L	concentration -	concentration -	concentration -

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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