M6018

4-Methylumbelliferyl β-D-cellobioside

glucanase substrate, fluorogenic, ≥98.00% (TLC), powder

• Synonym(s): 4-Methylumbelliferyl beta-D-cellobioside
• Empirical Formula (Hill Notation): C₂₂H₂₈O₁₃
• CAS Number: 72626-61-0
• Molecular Weight: 500.45
• EC Number: 276-744-7
• MDL number: MFCD00063279
• UNSPSC Code: 12352204
• PubChem Substance ID: 24897067
• NACRES: NA.32

Properties

• product name: 4-Methylumbelliferyl β-D-cellobioside, glucanase substrate
• Quality Level: 200
• Assay: ≥98.00% (TLC)
• form: powder
• solubility: water: 19.60-20.40 mg/mL, clear, colorless to faintly yellow
• storage temp.: 2-8°C
• SMILES string: CC1=CC(=O)Oc2cc(O[C@@H]3O[C@H](CO)[C@@H](O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)ccc12
• InChI: 1S/C22H28O13/c1-8-4-14(25)32-11-5-9(2-3-10(8)11)31-21-19(30)17(28)20(13(7-24)34-21)35-22-18(29)16(27)15(26)12(6-23)33-22/h2-5,12-13,15-24,26-30H,6-7H2,1H3/t12-,13-,15-,16+,17-,18-,19-,20-,21-,22+/m1/s1
• InChI key: PRTGXBPFDYMIJH-MKQZUAMYSA-N

Description

Application

4-Methylumbelliferyl β-D-cellobioside has been used as a substrate:

• to assay cellulase from A. niger
• to determine the extracellular enzyme activity for 1,4-β-cellobiohydrolase
• to assay the activity of bound enzyme, endoglucanase I (EGIc) Trichoderma reesei class II hydrophobins (HFBI)

Biochem/physiol Actions

4-Methylumbelliferyl β-D-cellobioside is used as a substrate for cellulase assays. It is mainly used for activity staining of cellulases in polyacrylamide gels.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves