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L3045

Sigma-Aldrich

β-Lactamase from Pseudomonas fluorescens

lyophilized powder, recombinant, expressed in E. coli

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About This Item

Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

form

lyophilized powder

enzyme activity

7.5-15 units/mg solid

shipped in

wet ice

storage temp.

2-8°C

Unit Definition

One unit will hydrolyze 1.0 μmole of benzylpenicillin per minute at pH 7.0 at 25 °C

Physical form

Lyophilized powder containing potassium phosphate and sodium bicarbonate

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Teerawit Tangkoskul et al.
The Southeast Asian journal of tropical medicine and public health, 43(6), 1461-1469 (2013-02-19)
We performed extended-spectrum beta-lactamase (ESBL) phenotypic testing and molecular characterization of three ESBL genes (TEM, SHV and CTX-M) and susceptibility testing by Clinical Laboratory Standards Institute (CLSI) disk diffusion method against three cephalosporins (ceftriaxone, ceftazidime, cefepime) and a cephamycin (cefoxitin)
MMWR. Morbidity and mortality weekly report, 62(6), 108-108 (2013-02-15)
On August 16, 2012, the Colorado Department of Public Health and Environment was notified of two patients at an acute-care hospital in Denver with carbapenem-resistant Enterobacteriaceae (CRE), specifically Klebsiella pneumoniae (CRKP), isolated from respiratory specimens during July-August. Both isolates produced
Grace Yim et al.
Canadian journal of microbiology, 59(2), 110-116 (2013-03-07)
Microbial populations in wastewater treatment plants (WWTPs) are increasingly being recognized as environmental reservoirs of antibiotic resistance genes. PCR amplicons for plasmid-mediated quinolone resistance determinants qnrA, qnrB, and qnrS were recorded in samples from a WWTP in Vancouver, British Columbia.
Siriluck Anunnatsiri et al.
The Southeast Asian journal of tropical medicine and public health, 43(5), 1169-1177 (2013-02-26)
Escherichia coli producing extended spectrum beta-lactamase (ESBL) has emerged as a worldwide, public health problem. The aims of this study were to determine the incidence of ESBL-producing E. coli septicemia and evaluate the factors associated with the infection and the
Seung Bok Hong et al.
Journal of medical microbiology, 62(Pt 6), 836-844 (2013-03-23)
The co-existence of carbapenemase, 16S rRNA methylase and mutated quinolone resistance-determining regions (QRDRs) can cause serious difficulty in treating infections with multidrug-resistant Acinetobacter baumannii. In this study, we aimed to determine the mechanisms of imipenem, amikacin and ciprofloxacin resistance in

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