I0408

Invertase Glycoprotein Standard

BioReagent, from Saccharomyces cerevisiae, for proteomics

• Synonym(s): Invertase from baker’s yeast (S. cerevisiae), β-D-Fructofuranosidase, β-D-Fructofuranoside fructohydrolase, Saccharase
• CAS Number: 9001-57-4
• Enzyme Commission number: 3.2.1.26 (BRENDA, IUBMB)
• EC Number: 232-615-7
• MDL number: MFCD00131410
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: Saccharomyces cerevisiae
• Quality Level: 200
• grade: for proteomics
• product line: BioReagent
• form: lyophilized powder
• mol wt: 60 kDa
• concentration: ≥0.5 mg/vial protein (E1%/280)
• storage temp.: 2-8°C

Description

Application

Invertase Glycoprotein Standard has been used:

• to generate N-linked glycan library
• as a negative control to study the binding of lectins to high mannose structures
• for sample pre-treatment in proteomic analyses to study drug-induced toxic epidermal necrolysis

The Invertase Glycoprotein Standard can be used to demonstrate N-glycosylation using PNGase F with both in-solution and in-gel procedures. The extent of deglycosylation can be assessed by mobility shift on SDS-PAGE gels.

Used in the production of confectionary foods and artificial honey.

Biochem/physiol Actions

Invertase hydrolyzes sucrose into glucose and fructose yielding a colorless product, unlike acid hydrolysis which produces colored products.

Other Notes

Invertase is an enzyme that catalyses the hydrolysis of sucrose into fructose and glucose. Invertase Glycoprotein Standard is the periplasmic (glycosylated form, external invertase) with 50% of its mass as polymannan. Since yeast can provide an alternative system for protein glycosylation that is similar to mammalian systems, periplasmic invertase is often used as a model for the study of the function of oligosaccharides in glycoproteins and for studies on glycoprotein biosynthesis.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves