GE17-0300-10
Ficoll® PM 400
Cytiva 17-0300-10, pack of 100 g
Synonym(s):
Density gradient medium
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About This Item
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form
solid
spray-dried
packaging
pack of 100 g
manufacturer/tradename
Cytiva 17-0300-10
color
White
solubility
soluble
Related Categories
General description
High molecular weight sucrose-polymers formed by copolymerization of sucrose with epichlorohydrin. The molecules are highly branched and the high content of hydroxyl groups leads to very good solubility in aqueous media.
It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be attained
Features and Benefits
- For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.
- Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution.
- Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/mL can be achieved.
- Better osmotic properties than sucrose.
- Preserves functional and morphological integrity.
- Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size (under conditions of sedimentation at unit gravity).
- Does not penetrate biological membranes.
- Serves as the raw material for preparation of Ficoll-Paque gradients.
- Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier.
Storage and Stability
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Ficoll is a registered trademark of Cytiva
Storage Class Code
13 - Non Combustible Solids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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