The concentration and volume of this product varies from lot to lot. The protein content and activity of this enzyme are reported on the lot-specific Certificate of Analysis. The volume may be calculated using this information. For example, the 200 unit package size of a given lot that reports a protein content of 2 mg/mL and an activity of 600 units per milligram protein will have a volume of 167 uL. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/US/en/product/sigma/g5760#product-documentation
G5760
Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
Type XXIII, ammonium sulfate suspension, 550-1,100 units/mg protein (biuret), ≥2.0 mg/mL Biuret
Synonym(s):
G-6-P-DH
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About This Item
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biological source
bacterial (Leuconostoc mesenteroides)
Quality Level
type
Type XXIII
form
ammonium sulfate suspension
specific activity
550-1,100 units/mg protein (biuret)
mol wt
54 kDa
concentration
≥2.0 mg/mL Biuret
foreign activity
6-Phosphogluconic dehydrogenase, hexokinase, NADH oxidase and NADPH oxidase ≤0.005%
PGI ≤0.01%
storage temp.
2-8°C
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General description
Research area: Cell Signaling
Glucose-6-phosphate dehydrogenase (G6PD) is found in the cytoplasm of all cells. The G6PD enzyme is encoded by the Gd gene, located on the long arm of the X chromosome.[1]Glucose-6-phosphate Dehydrogenase (G6PD) from Leuconostoc mesenteroides catalyzes the oxidation of glucose 6-phosphate in the presence of nicotinamide adenine dinucleotide (NAD+) or nicotinamide adenine dinucleotide phosphate (NADP+).[2] It corresponds to a molecular weight of 54 kDa and exists as a homodimer.[3]
Glucose-6-phosphate dehydrogenase (G6PD) is found in the cytoplasm of all cells. The G6PD enzyme is encoded by the Gd gene, located on the long arm of the X chromosome.[1]Glucose-6-phosphate Dehydrogenase (G6PD) from Leuconostoc mesenteroides catalyzes the oxidation of glucose 6-phosphate in the presence of nicotinamide adenine dinucleotide (NAD+) or nicotinamide adenine dinucleotide phosphate (NADP+).[2] It corresponds to a molecular weight of 54 kDa and exists as a homodimer.[3]
Application
Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used:
- as a component of reaction mixture for assaying mannose- and glucose-6-phosphate activity[4]
- as a component of reaction mixture in the nevirapine inhibition studies in human hepatic microsomes[5]
- in the glucose-phosphorylating activity of chloroplast extracts[6]
- as a model to test the effect of seed protein fractions on enzyme protection during dehydration.[7]
- in assays for nicotinamide adenine dinucleotide and tissue pyridine nucleotides.[8]
Biochem/physiol Actions
Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.
Glucose-6-phosphate dehydrogenase (G6PD) is a housekeeping enzyme that protects cells from damage caused by reactive oxygen species by supplying substrates that help prevent oxidative harm.[1] G6PD from Leuconostoc mesenteroides catalyzes the oxidation of glucose 6-phosphate in the presence of nicotinamide adenine dinucleotide (NAD+) or nicotinamide adenine dinucleotide phosphate (NADP+) [2] as the first step in the pentose phosphate pathway. Lysine 21 in the Glucose-6-phosphate Dehydrogenase (G6PD) is crucial for binding NAD+.[2] G6PD uses NAD+ or NADP+ based on the catabolic or anabolic metabolic pathway.[3]G6PD deficiency can lead to acute hemolysis, neonatal jaundice, or severe chronic non-spherocytic hemolytic anemia.
Unit Definition
One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.
Physical form
Suspension in 2.7 M (NH4)2SO4 solution containing 42 mM Tris and 0.8 mM MgCl2
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Customers Also Viewed
Glucose-6-Phosphate Dehydrogenase Deficiency
Richardson SR and O'Malley GF
StatPearls [Internet] (2022)
Oligomerization studies of Leuconostoc mesenteroides G6PD activity after SDS-PAGE and blotting
Ravera S, et al.
Molecular Biology, 44(3), 415-419 (2010)
In vitro studies: inhibition of nevirapine metabolism by nortriptyline in hepatic microsomes
Usach I and Peris JE
Bio-protocol, 5(19) (2014)
A novel type of chloroplast stromal hexokinase is the major glucose-phosphorylating enzyme in the moss Physcomitrella patens
Olsson T, et al.
The Journal of Biological Chemistry, 278(45), 44439-44447 (2003)
A Modified Method for Detection of Post-Phoretic Activities in Mannose-(Mpi, EC 5.3. 1.8) and Glucose-(Gpi, EC 5.3. 1.9) 6-Phosphate Isomerases
Zasypkin MY, et al.
Russian Journal of Genetics, 37(5), 581-583 (2001)
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