G0251
β-Glucuronidase from bovine liver
Type B-1, ≥1,000,000 units/g solid
Synonym(s):
Bovine Liver beta-Glucuronidase, β-D-Glucuronide glucuronosohydrolase
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General description
β-Glucuronidase (GUSB) is a lysosomal enzyme, found in animals, plants and bacteria. This exoglycosidase shows high activity in spleen, liver and kidney. Bovine liver β-glucuronidase (BLG) is homotetramer with a molecular weight of 290 kDa.
Application
β-Glucuronidase from bovine liver has been used in β-glucuronidase inhibition assay to study the β-glucuronidase inhibitory effect of extracts and isolated actives from Phyllanthus amarus. It has also been used to perform deglucuronidation.
β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.
Biochem/physiol Actions
β-Glucuronidase (GUSB) deficiency can result in mucopolysaccharidosis type VII (MPSVII), which leads to store lysosome in the brain. Integration of β-glucuronides of steroid hormones into tissues can be done with the help of GUSB.
β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.
Unit Definition
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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