F0137

Fructose-6-phosphate Kinase from Bacillus stearothermophilus

Type VII, lyophilized powder, ≥50 units/mg protein

• Synonym(s): 6-Phosphofructokinase, ATP:D-fructose 6-phosphate 1-phosphotransferase
• CAS Number: 9001-80-3
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-633-5
• MDL number: MFCD00131107
• EC Number: 2.7.1.11 (BRENDA, IUBMB)
• Specific activity: ≥50 units/mg protein

Properties

• type: Type VII
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥50 units/mg protein
• mol wt: 34 kDa
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Research Area: Cell Signaling
Bacillus stearothermophilus phosphofructokinase (BsPFK) is a homotetramer that is allosterically inhibited by phosphoenolpyruvate (PEP), which binds along one dimer-dimer interface.

Fructose-6-phosphate kinase from Bacillus stearothermophilus, a 34 kDa enzyme, belongs to phosphofructokinase (PFK) - like superfamily. The glutamate 161 and arginine 162 residues at the active site are crucial for substrate binding.

Application

Fructose-6-phosphate Kinase from Bacillus stearothermophilus was shown to interact with neuronal nitric oxide synthase (nNOS) causing a defect in glycolytic metabolism and increased fatigability in dystrophic muscle.

Fructose-6-phosphate Kinase from Bacillus stearothermophilus has been used in the assay mixture for mass spectrometry assay for phosphoglucoisomerase (PGI) (G6P to F6P reaction).

Fructose-6-phosphate kinase from Bacillus stearothermophilus has been used:

• in steady state analysis of phosphofructokinase activity in the presence of ATP deuterated at the C8 position(C8-D ATP)
• for standard curve generation for quantifying muscle phosphofructokinase (PFK) activity

Biochem/physiol Actions

Fructose-1,6-bisphosphatase (FBP) is an important enzyme in glucose metabolism. It catalyzes the hydrolysis of fructose-1,6-bisphosphate to fructose-6-phosphate and inorganic phosphate. Fructose-6-phosphate kinase converts fructose-6-phosphate into fructose 1,6-bisphophate in the rate limiting step of the glycolysis cycle.

Phosphofructokinase (PFK) is an essential bifunctional enzyme that serves as a critical regulator in the intermediate stages of glycolysis. PFK is strongly linked to caveolae and is brought to caveolae by caveolin-1 in vascular smooth muscle cells (VSMCs).

Physical form

Lyophilized powder containing buffer salt (e.g. phosphate buffer, or Tris buffer with NaCl)

Other Notes

One unit will convert 1.0 μmole of fructose 6-phosphate and ATP to fructose 1,6-diphosphate and ADP per minute at pH 9.0 at 30 °C.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)