U-2 OS Cell Line human
92022711, human bone, Not specified
Synonym(s):
U-2OS Cells, U2-OS Cells, U20S Cells, U2OS Cells
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About This Item
UNSPSC Code:
41106514
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product name
U-2 OS Cell Line human, osteosarcoma, 92022711
biological source
human bone
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Osteosarcoma
Cell Line Description
Cell line derived in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.
Application
U-2 OS has been used to study:
- the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA)
- the interaction of human single-stranded DNA binding protein 1 (hSSB1) with bloom syndrome protein helicase (BLM helicase)
- calcium-mediated actin reset (CaAR) in response to physiological changes
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 13
D16S539: 11,12
D5S818: 11
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 14,18
CSF1PO: 13
D13S317: 13
D16S539: 11,12
D5S818: 11
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 14,18
Culture Medium
McCoy′s 5a medium with 1.5 mM Glutamine; 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split subconfluent 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new culture flasks.
Other Notes
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Certificates of Analysis (COA)
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