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I-10

89031605, mouse testis, Epithelial

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About This Item

UNSPSC Code:
41106514

product name

I-10, 89031605

biological source

mouse testis

growth mode

Adherent

karyotype

2n = 40, hyperdiploid

morphology

Epithelial

products

Steroids

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

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This Item
22641655201444286
solubility

THF: soluble

solubility

water: soluble(lit.)

solubility

H2O: 1 g/10 mL, clear, colorless to almost colorless

solubility

-

bp

78-81 °C/10 mmHg (lit.)

bp

86-88 °C/12 mmHg (lit.)

bp

-

bp

133 °C/10 mmHg (lit.)

refractive index

n20/D 1.432 (lit.)

refractive index

n20/D 1.472 (lit.)

refractive index

-

refractive index

n20/D 1.467 (lit.)

Cell Line Origin

Mouse BALB/c Leydig cell testicular tumour

Cell Line Description

Derived from a Leydig cell testicular tumour of a BALB/c mouse. The cells secrete steroids; this increases at saturation density. Secretion should be retained for over 50 passages. Exposure to 0.00001M cAMP stimulates secretion 4-5 fold. The major products are progesterone and its derivative 20 α-hydroxy-4-pregnen-3-one. It does not respond to interstitial cell stimulating hormone (ICSH) but is stimulated by cyclic adenosine monophosphate (AMP).

Application

Tumourigenicity studies

Culture Medium

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 6x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.

Other Notes

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Certificates of Analysis (COA)

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