B3804
BCIP®/NBT-Blue
alkaline phosphatase substrate, chromogenic, liquid
Synonym(s):
5-Bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium liquid substrate system
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.83
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product name
BCIP®/NBT-Blue Liquid Substrate System for Membranes, alkaline phosphatase substrate
Quality Level
form
liquid
storage temp.
2-8°C
Related Categories
General description
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-bromo4-chloro-3-indolyl phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end-product that is blue to purple and can be observed visually. BCIP®/NBT-blue liquid substrate system for membranes is a specifically formulated liquid enzyme substrate that is stable and optimized for blotting applications offers maximum convenience, time savings, and consistency.
Application
BCIP®/NBT-Blue Liquid Substrate System for Membranes has been used in colorimetric detection of alkaline phosphatase (ALP) activity such as western blot analysis.
Caution
Stable at least one year at 2-8 °C. This substrate is light-sensitive.
Other Notes
Not recommended for microwell (e.g., ELISA) or immunohistochemical applications.
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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