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Sigma-Aldrich

Atto 390 azide

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

Empirical Formula (Hill Notation):
C28H41N5O6
Molecular Weight:
543.66
UNSPSC Code:
12352125
PubChem Substance ID:
NACRES:
NA.32

product line

BioReagent

Quality Level

Assay

≥90% (HPLC)

form

solid

manufacturer/tradename

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 380-384 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CC(CC(C)(C)N1CCCC(NCCOCCOCCOCCN=[N+]=[N-])=O)C2=C1C=C(OC(C=C3C)=O)C3=C2

InChI

1S/C28H41N5O6/c1-20-16-27(35)39-25-18-24-22(17-23(20)25)21(2)19-28(3,4)33(24)9-5-6-26(34)30-7-10-36-12-14-38-15-13-37-11-8-31-32-29/h16-18,21H,5-15,19H2,1-4H3,(H,30,34)

InChI key

FEBSRHTZZIMYNR-UHFFFAOYSA-N

General description

Atto 390 is a fluorescent label with a coumarin structure. The dye is intended for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are high fluorescence quantum yield, large Stokes-shift, good photostability and low molecular weight.
The azide modification is suitable for reactions with alkyne groups (Huisgen reaction - “Click Chemistry“).

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Qiang Li et al.
The journal of physical chemistry. B, 115(46), 13643-13649 (2011-10-15)
Fluorescence resonance energy transfer (FRET) using biotinylated β-galactosidase (βGAL) as a donor and Alexa Fluor 350 (AF350) labeled avidin as an acceptor has been investigated by means of steady-state fluorescence and time-resolved fluorescence spectroscopy. The donors are readily paired with
Cuifang Kuang et al.
The Review of scientific instruments, 81(5), 053709-053709 (2010-06-03)
Stimulated emission depletion (STED) microscopy is one of the breakthrough technologies that belong to far-field optical microscopy and can achieve nanoscale spatial resolution. We demonstrate a far-field optical nanoscopy based on continuous wave lasers with different wavelengths, i.e., violet and
Simon Corrie et al.
Molecular bioSystems, 5(3), 262-268 (2009-02-20)
Herein we report a method for the detection of methylated CpG dinucleotides located within CpG islands in genomic DNA using multiplexed bead-based assays and standard flow cytometry instrumentation. Four CpG "clusters" were identified in the TFPI2 and SPARC CpG islands
Philip Howes et al.
Journal of the American Chemical Society, 132(11), 3989-3996 (2010-02-24)
Semiconducting polymer nanospheres (SPNs) have been synthesized and encapsulated in phospholipid micelles by a solvent evaporation technique. Four different conjugated polymers were used, yielding aqueous dispersions of nanoparticles which emit across the visible spectrum. The synthesis was simple and easily
Gerhild Zauner et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 13(25), 7085-7090 (2007-06-20)
A fluorescence-based system to sense oxygen in solution is described. The method exploits the sensitivity of the endogenous fluorescence of type-3 copper proteins towards the presence of oxygen by translating the near-UV emission of the protein to label fluorescence in

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