R1273

Rose bengal agar base

suitable for microbiology, NutriSelect^® Plus

• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• pH: 7.2±0.2 (25 °C)
• application(s): bioburden testing; clinical testing; environmental; food and beverages; microbiology
• suitability: selective for Aspergillus spp.; selective for Candida spp.; selective for Penicillium spp.; selective for Pichia spp.; selective for Saccharomyces spp.; selective for Zygosaccharomyces spp.; selective for molds (General Media); selective for yeasts (General Media)

Description

Application

Ingredients (g/L) for the selective isolation and enumeration of yeasts and molds.

Components

Ingredients (g/L)
Papic digest of soybean meal, 5.00
Dextrose, 10.00
Monopotassium phosphate, 1.00
Magnesium sulphate, 0.50
Rose bengal, 0.05
Agar, 15.00

Preparation Note

Suspend 31.55 g of Rose Bengal Agar Base in 1000 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 45°C and add 2 vials of rehydrated Chloramphenicol Selective Supplement (Cat. No. 29231) to 1 litre of the Rose Bengal Agar Base. Mix thoroughly and pour into sterile petri plates.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves